漆酶/TEMPO体系氧化棉纤维接枝壳聚糖制备抗菌纤维

摘要

利用漆酶/TEMPO 体系选择性将纤维素C-6 伯羟基氧化成醛基,得到单醛纤维素,再将壳聚糖通过席夫碱反应接枝到单醛纤维素上,制得接枝产物.将接枝产物进行红外光谱分析、SEM及能谱分析及壳聚糖含量的分析,确定接枝产物结构的变化和壳聚糖含量,并进一步对其抗菌性进行研究.结果表明,经漆酶/TEMPO 体系氧化后,纤维素 C-6 伯羟基被选择性的氧化成醛基,与壳聚糖接枝反应后,壳聚糖的氨基与醛基反应生成C ?N;接枝产物表面出现了明显的变化且有壳聚糖存在;接枝产物中壳聚糖含量为 4.55%;与棉纤维相比,接枝产物对大肠杆菌和金黄色葡萄球菌均具有抗菌性,且对大肠杆菌的抑制作用强于金黄色葡萄球菌.%The laccase/TEMPO system was used to selectively oxidize the C-6-hydroxyl groups from the cotton fibers into aldehyde groups to generate monoaldehyde cellulose.And then the chitosan was grated on the mono-aldehyde cellulose via Schiff base reaction to obtain the grafted product.The structures and property of the graf-ted product were characterized by FT-IR,SEM,energy spectrum analysis,the chitosan content analysis and antibacterial experiment.Results showed that the C-6-hydroxyl of cellulose was selectively oxidized to aldehyde groups.After the Schiff base reaction,the amino groups of chitosan reacted with aldehyde groups to form C(=)N.The surface of the cotton fiber changed dramatically and the chitosan was present on the surface of the cotton fiber.The content of chitosan in the grafted product was 4.55%.The grafted products had antibacterial activity against escherichia coli and staphylococcus aureus compared with cotton fiber.The inhibitory effect of grafted products on Escherichia coli was stronger than that of Staphylococcus aureus.