Objective:To study the effect of total saponins of panax notoginseng (PNS) on high-fat diet induced lipids deposition on the aortic tissue of apolipoprotein E-knockout (Apo E-/-) mice. Methods:The atherosclerosis models were prepared with a high-fat diet in the Apo E-/-mouse model for 12 weeks. All Apo E-/-mice were randomly divided into three groups (model group, simvastatin group and total saponins group), and the wild type C57 BL/6 J mice were taked as the control group. Apo E-/-mice continuely intook high fat feeding by gavage for 8 weeks. Blood and the aortic root tissue were collected and serum lipid level was detected by biochemical analyzer, the serum IL-1β, IL-10 concentration was detected by ELISA, the lipid deposition of aortic tissue was observed by oil red O staining, CD+68 macrophage aggregation of aortic tissue was observed by immunohistochemical staining. Results:In the model group, TC and LDL-C were significantly increased, HDL-C was slightly reduced, and IL-1β, IL-10 were also increased significantly (P＜0.05). Aortic tissue oil red O positive staining cells increased significantly (P＜0.05), plaque lipid deposition was evident. The dots or patches protrude into the vascular and the vascular cavity was markedly narrowed, CD+68 macrophage was also increased significantly in aortic tissue (all P＜0.05). Compared to the model group, TC, LDL-C and IL-1β, IL-10 were significantly reduced (P＜0.05), HDL-C was slightly increased in both the simvastatin group and the total saponins group (P＜0.05). Positive staining cells of oil red O in aortic tissue, were significantly reduced, plaque lipid deposition and the area decreased. The degree of convexity was significantly reduced. CD+68 macrophage number and the macrophage aggregation were significantly reduced (all P＜0.05).Conclusion:PNS can regulate blood lipids, inhibit the immune inflammatory response, inhibit the lipid deposition of aortainhibiting and macrophage aggregation, which might be one of the anti-atherosclerosis mechanisms.%目的:研究三七总皂苷对高脂饮食诱导的载脂蛋白E基因敲除(Apo E-/-)小鼠主动脉组织脂质沉积的影响.方法:以高脂饮食喂养Apo E-/-小鼠12周制作动脉粥样硬化模型,按照随机数字表法分为辛伐他汀组、三七总皂苷组和模型组,每组5只,以野生型C57BL/6J小鼠作为对照组;Apo E-/-小鼠继续高脂喂养并灌胃给药8周后,取血及主动脉根部组织,采用生化测定仪检测血脂水平,ELISA检测血清中白细胞介素IL-1β、IL-10水平,油红O染色观察主动脉组织脂质沉积情况,免疫组织化学染色观察主动脉组织CD68+巨噬细胞聚集情况.结果:高脂诱导的模型组小鼠血清TC、LDL-C显著升高,HDL-C有所降低,IL-1β、IL-10显著升高,主动脉组织油红O阳性染色细胞显著增多,斑块脂质沉积明显,可看到较明显的点状或斑片状斑块突向管腔,管腔狭窄明显,主动脉组织CD68+巨噬细胞显著增多,可看到较明显的巨噬细胞聚集,与其他各组比较,差异均有统计学意义(P＜0.05).与模型组相比,三七总皂苷组和辛伐他汀组小鼠血清中的TC和LDLC均显著降低,而HDL-C显著升高,IL-1β、IL-10显著降低,主动脉组织中油红O阳性染色细胞明显减少,斑块脂质沉积减少,面积减小,凸向管腔程度明显降低,CD68+巨噬细胞明显减少,聚集程度显著减低,差异均有统计学意义(P＜0.05).结论:三七总皂苷可以调节血脂,抑制免疫炎症反应,抑制主动脉组织脂质沉积和巨噬细胞聚集,这可能是其抗动脉粥样硬化的作用机制之一.
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