Objective To evaluate the effects of Indoleamine 2 ,3-dioxygenase (IDO) on the mice allogeneic hepatocytes transplanted into spleen . Methods BABL/c mice hepatocytes were isolated and transplanted into C57BL/6 mouse and divided into IDO group (I group ) , IDO + 1-methyl tryptophan (1-MT) group(Ⅱ group) ,shell adenovirus group(Ⅲ group) and hepatocyte group(Ⅳ group) . The IDO was detected by immunohistochemistry and lymphocyte apoptosis of spleen were detected by TUNEL on the day 2 ,5 after transplantation . Results Hepatocytes effectively expressed IDO after transfection . Immunohistochemistry showed that IDO-positive hepatocytes were observed in Ⅰ and Ⅱ group . Com-pared with other groups ,the difference of lymphocyte apoptosis in Ⅰ group was statistically significant on the day 2 ,5 after transplantation(P<0 .05) ,no difference were observed between Ⅱ ,Ⅲ and Ⅳ groups (P>0 .05) . Conclusion Mice allogeneic hepatocytes transfected with IDO can stably express IDO and induce lymphocyte apoptosis in spleen .%目的：研究携带吲哚胺2，3-二氧化酶（IDO）的小鼠肝细胞在同种异体脾内的表达及对脾淋巴细胞的凋亡作用。方法分离BABL/c小鼠肝细胞，肝细胞脾内移植（BABL/c→C57BL/6），分为4组：Ⅰ组为携带IDO组；Ⅱ组为1-甲基色氨酸组；Ⅲ组为空壳腺病毒组；Ⅳ组为单纯肝细胞移植组。移植术后第2，5d取脾脏免疫组织化学检测IDO表达情况，TUNEL检测脾脏淋巴细胞凋亡情况。结果携带IDO腺病毒转染肝细胞后，能有效表达IDO。免疫组织化学显示，Ⅰ、Ⅱ组脾脏有IDO阳性的肝细胞。与其他组比较，移植术后第2，5dⅠ组淋巴细胞凋亡差别有统计学意义（P＜0．05），Ⅱ、Ⅲ、Ⅳ组之间比较，差别无统计学意义（P＞0．05）。结论携带IDO的肝细胞能在小鼠同种异体脾内稳定表达，并能诱导脾内淋巴细胞的凋亡。
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