Objective To detect the expression of Ezrin protein in gastric cancer and investigate its association with the clinicopathologic features and its influence on clinical prognosis ;To explore the mech‐anism of the effect of Ezrin protein on the grow th and invasiveness in gastric cancer , preliminarily . Methods 206 cases of gastric cancer tissues without peritoneal metastasis ,34 cases of peritoneal metastatic tissues and 54 cases of adjacent non‐cancerous tissues of paraffin‐embedded specimens with detailed clinico‐pathological parameters from December 2006 to September 2007 were collected from Fujian Medical Uni‐versity Union Hospital . On these samples ,immunohistochemical SP method was used to detect the ex‐pression level of Ezrin and CD44v6 . Western‐blot assay was used to detect the expression level of Erin in 16 cases of gastric cancer tissues and its adjacent non‐cancerous tissues ,which were collected after surgery from May to June of 2012 . The association of Ezrin expression with clinicopathological parameters and with CD44v6 expression were analyzed with SPSS 17 .0 software . Results The rate of Ezrin protein ex‐pression in gastric cancer tissues was considerably higher than that in adjacent non‐cancerous tissues (P<0 .05) . The rate of Ezrin protein expression in peritoneal metastatic tissues was considerably higher than that in primary gastric cancer tissues (P<0 .05) . Ezrin protein relative expression intensity in gas‐tric cancer tissues was significantly higher than its adjacent non‐cancerous tissues (P<0 .05) . Cell func‐tion experiments showed that the cell growth rate was significantly inhibited (P<0 .05) and the cell inva‐sion rate was significantly lower than that in the control group (P<0 .05) ,if we downregulated the ex‐pression of Ezrin protein . The higher expression of Ezrin protein in gastric cancer was associated with lymphatic metastasis . Overexpression of Ezrin protein had a significant correlation with poor survival in prognostic analyses (P<0 .05) . The rate of CD44v6 expression in gastric cancer tissues was considerably higher than that in adjacent non‐cancerous tissues (P< 0 .05) . The expression of Ezrin protein and CD44v6 was significantly positively correlated (P<0 .05) . Conclusions These results indicate that Ezrin protein was overexpressed in gastric cancer ;downregulated expression of Ezrin protein contributed to inhi‐bition in the invasion and metastasis of gastric cancer ;Ezrin was significantly associated with lymph node metastasis and clinical prognosis ;Ezrin protein may affect the grow th and invasiveness of gastric cancer through CD44v6 .%目的：分析Ezrin蛋白与胃癌患者临床病理参数之间的关系并探讨其预后价值，初步探讨Ezrin蛋白在胃癌增殖侵袭中的作用机制。方法收集无腹膜转移的胃癌组织206例、腹膜转移灶癌组织34例及癌旁组织54例的石蜡标本，采用免疫组织化学方法检测 Ezrin蛋白和CD44v6的表达情况；同时收集新鲜胃癌组织及其癌旁组织16例，采用Western‐blot方法检测Ezrin蛋白的表达情况。应用细胞功能学实验及RNA干扰技术研究Ezrin蛋白在胃癌侵袭过程中的作用，分析Ezrin蛋白与CD44v6的相关性。结果 Ezrin蛋白强阳性表达率胃癌组织明显高于癌旁组织，腹膜转移灶癌组织高于原发胃癌组织（均为 P＜0．05）。在16例新鲜胃癌组织中，Ezrin蛋白相对表达强度明显高于邻近癌旁组织（P＜0．05）。细胞功能实验显示，Ezrin蛋白表达下调组细胞的生长速率胃癌组织明显受到抑制（P＜0．05），细胞侵袭能力均明显降低（P＜0．05）。Ezrin蛋白高表达与患者的淋巴结转移显著相关（P＜0．05）；Ezrin蛋白强阳性表达的患者5年生存率明显低于弱阳性表达的患者（P＜0．05）。癌旁组织中CD44v6呈低表达，而在胃癌组织中呈高表达，而且CD44v6表达与Ezrin蛋白呈显著正相关（P＜0．05）。结论Ezrin蛋白在胃癌中的表达上调，下调Ezrin蛋白能显著抑制细胞的增殖和侵袭能力；Ezrin与胃癌淋巴结转移及患者预后显著相关；Ezrin蛋白可能通过CD44v6影响胃癌增殖侵袭过程。
展开▼
