载紫杉醇聚合物胶束的制备工艺优化

摘要

目的 优化载药胶束的制备工艺,并对其稳定性进行考察. 方法 采用高效液相(HPLC)法测定紫杉醇(PTX)含量,以载药量、包封率、粒径为考察指标,通过单因素考察方法优化载药胶束的制备工艺. 结果优化工艺下制备的载PTX胶束载药量为(38.63 ± 0.42)%,包封率为(83.19 ± 1.23)%,粒径为(192.2 ± 0.5)nm,载PTX聚合物胶束一定条件下贮存10 d后,粒径与载药量无明显变化. 结论 该载药工艺简单可行,可用于载PTX聚合物胶束的制备,所制备的聚合物胶束短期贮存稳定.%Objective To optimize the preparation process of PTX-loaded polymer micelles. Study on the stability of polymer micelles. Methods The HPLC activity was used to determine the con-tent of PTX. By using drug loading content,encapsulation efficiency and particle size as assessment inde-xes,the drug-loading process was optimized through the single factor investigation. Results The drug loading efficiency(DL),entrapment efficiency(EE)and average particle size of PTX-loaded polymer mi-celles were(38.63 ± 0.42)%,(83.19 ± 1.23)% and(192.2 ± 0.5)nm. There was no significant differ-ences in size and drug loading of the paclitaxel polymer micelles for 10 days. Conclusion T he drug-load-ing process was simple and feasible,and can be used to prepared the PTX-loaded polymer micelles. The prepared polymer micelles are stable for short-term storage.