福建山樱花组织培养试验

摘要

以半木质化枝条为外植体，探讨外植体消毒时间、植物生长调节剂对福建山樱花组培各阶段的影响，结果表明：外植体先用75％酒精消毒30 s，再使用0.1％HgCl溶液消毒5 min的消毒效果较好。较适宜的诱导培养基为：MS＋6-BA 1.5 mg· L-1＋NAA 0.1 mg· L-1；较适宜的继代培养基为：3/2 MS＋6-BA 1.0 mg· L-1＋NAA 0.2 mg· L-1；较适宜的生根培养基为：1/3 MS＋IBA 1.0 mg· L-1＋NAA 0.2 mg· L-1，生根率达97.4％。生根苗移栽到泥炭土∶珍珠岩∶黄心土（6∶3∶1）的基质中成活率可达90％以上。%Taking half lignification stems as explants ,this article discusses about the impact of sterilization time and plant growth reg-ulator for Cerasus campanulata Maxim in each stage.The results showed that:the best method for sterilization of explant was 75%of ethanol for 30 s first then 0.1%of HgCl for 5 min.The optimum medium for continue generation subculture of C.campanulata Max-im was 3/2 MS+6-BA 1.0 mg· L-1 +NAA 0.2 mg· L-1.Medium 1/3 MS+IBA 1.0 mg· L-1 +NAA 0.2 mg· L-1 was relative suitable rooting medium for C.campanulata Maxim,with a rooting rate of 97.4%.The survival rate of seedlings could reach above 90%in substrate of peat soil∶perlite∶yellow soil=6∶3∶1.