A full-length cDNA library from the pericarp of Minima 6 peanut was constructed based on switching mechanism at 5'end of RNA transcript (SMART) system. The purified double strand cDNA was ligated to Sif I digested vector pDNR-LIB, and transformed into DH5α by electroporation. The entry library constructed has a high titer of 1.3 x 107 cfu. PCR results showed that the inserts varied from 750 to 2000 bp with average size larger than 1000 bp and 95.5% of recombinant percentage. As results bioinforma-tics analysis, about 68% of sequences were full-length. Thirteen sequences with known functions were identified by BlastX searched against the NCBI non-redundant protein databases. These results will be useful for screening and cloning pericarp special expression genes and adding genetic information for peanut.%以闽花6号花生为材料,利用SMART技术构建了花生果皮全长cDNA文库.从花生果皮中分离、纯化的cDNA片段连接到经sfiI酶切的pDNR-LIB载体上,经电击转化获得原始文库,经过涂平板测定和酶切反应快速鉴定表明,原始文库滴度达1.3×107 cfu,重组率达95.5％,插入片段大小多为750-2000 bp,平均大小在1000 bp左右.随机挑选30个单克隆进行双向测序,获得有效序列25条,序列经GenBank检索和生物信息学比较,17个获得了全长,所占比例为68％.有13条序列与其他植物已知功能氨基酸序列具有较高的相似性.说明得到了高质量的全长cDNA文库,这为筛选和克隆花生果皮特异表达基因和分离果皮特异启动子提供了基础.
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