红花蜂花粉多糖抗肿瘤活性研究

摘要

目的：观察红花蜂花粉多糖(PBPC)对荷S180肉瘤小鼠的肿瘤抑制作用。方法：通过建立小鼠S180肉瘤模型，观察PBPC的体内抗肿瘤活性及对小鼠免疫器官的影响，用ELISA法测定荷瘤小鼠血清中肿瘤坏死因子α（TNF-α）的含量。结果：以50，100，150 mg•kg-1剂量的PBPC灌胃给药，连续14d,能明显抑制小鼠S180肉瘤生长，抑制率分别为36.99%、41.78%与49.32%。PBPC对小鼠的免疫器官具有保护作用，PBPC100，150 m g•k g-1的剂量能明显增加荷瘤小鼠的胸腺和脾腺指数。另外，PBPC 50，100，150 mg•kg-1剂量组均能使荷瘤小鼠血清TNF-α显著升高，TNF-α含量分别为314.62，357.34与363.42 pg.L-1。结论：PBPC能明显抑制小鼠S180肉瘤的生长，增加荷瘤小鼠的胸腺和脾腺指数及血清TNF-α含量。提示PBPC促进荷瘤小鼠血清TNF-α的分泌从而引起肿瘤的坏死，可能是其抗肿瘤作用的途径之一。%Objectives: To observe the anti-tumor activity of of polysaccharide from bee pol en of carthamus tinctorius(PBPC). Methods: The model of S180-bearing mice was established to study the anti-tumor activity and effect on immune organs of PBPC in vivo. Then the serum content of TNF-α was tested by ELISA method Results: With the dosages of 50, 100, 150 mg-kg-1 by ig administration for 14 days, PBPC could significantly inhibit the tumor growth, the inhibition rates were 36.99%、41.78% and 49.32% respectively. On the other hand, compared with the model control group, PBPC 50, 100, 150 mg-kg-1 dosages could markedly increase serum TNF-α in tumor-bearing mice, the concentration of TNF-α were 314.62,357.34 and 363.42 pg.L-1 respectively. In addition, PBPC could increased the thymus and spleen index. Conclusion: PBPC can significantly inhibit the tumor growth in S180-bearing mice, increase the serum TNF-α level. The effect of PBPCⅡ promotes secretion of TNF-α to enhance tumor necrosis may be one of the pathways for PBPC’s effect of anti-tumor.