三氧化二砷对肝癌HepG2细胞增殖的影响

摘要

AIM　To explore the inhibitive effect of arsenic trioxide (As2O3) on the proliferation of human liver cancer cell line-HepG2. METHODS　The morphologic changes, proliferation ability, colony-forming rate of HepG2 treated by As2O3 were studied with light microscopy, growth curve, trypan blue and colony-forming assay. The cytotoxicity of As2O3 on HepG2 was determined by MTT assay. RESULTS　Colony-forming rate of HepG2 with 5 μmol*L-1 As2O31.5±1.2)% was lower than that with 1 μmol*L-1 As2O3 (12.3±2.2)%, P<0.01 and much lower than that of control group (30.0±4.2)%, P<0.01. As2O3 had a remarkable cytotoxic effect on HepG2 and cytotoxic effect was dose-and-time dependent. The cytotoxic effect was enhanced through the combination of As2O3 with 5-Fu or MMC. CONCLUSION　As2O3 can significantly inhibit proliferation of HepG2 and so has a potential value of the clinical therapy on liver cancer.%目的研究三氧化二砷（As2O3）对肝癌细胞的增殖抑制作用. 方法应用台盼蓝拒染法、MTT比色法及细胞克隆形成试验，研究As2O3对肝癌HepG2细胞的增殖抑制和细胞毒作用. 结果 As2O3能显著抑制肝癌细胞的生长，5 μmol*L-1 As2O3抑制程度明显强于1 μmol*L-1. 5和1 μmol*L-1 As2O3作用后细胞克隆形成率分别为(1.5±1.2)%和(12.3±2.2)%，明显低于对照组的(30.0±4.2)% (P<0.01). As2O3对肝癌细胞有较强的细胞毒作用，且呈明显剂量和时间依赖性，其对肝癌细胞的杀伤率高于5-氟脲嘧啶（5-FU）和丝裂霉素（MMC），但无显著性差异，As2O3与5-FU及MMC存在协同效应，联合应用杀伤率明显升高. 结论 As2O3具有明显的抑制肝癌细胞增殖作用，有必要进一步探讨其对肝癌的治疗价值.