猪眼玻璃体液钾离子、次黄嘌呤浓度变化与PMI推断

摘要

Objective To investigate the relationship between postmortem interval (PMI) and concentration changes of components in swine vitreous humor. Methods Ninety-six porcine eyes from swine dying from acute massive hemorrhage, being randomly divided into 24 groups, were stored in dark situation, at temperature of (15+2)℃ and humidity of(50+5)％ for 2-96 hours separately. The vitreous humor was collected.Concentrations of K+, Na+, CI- and hypoxanthine(Hx) were analyzed by automatic biochemical analyzer and ultra performance liquid chromatograph (UPLC). The data were statistically analyzed by SPSS software.Results Linear regression analysis showed that concentrations of vitreous K+ and Hx were positively correlated with PMI(R2-0.767 and R2=0.793, respectively). Binary linear regression showed a higher correlation for K＋ and Hx with PMI estimation (R2=0.866). PMI was not significantly correlated with vitreous Na+ and C1- concentrations. Conclusion Vitreous K+ and Hx concentrations can be used as the objective markers for PMI estimation. The binary linear regression functions of vitreous K+ and Hx concentrations with PMI are more accurate for estimating the PMI.%目的 研究猪眼玻璃体液成分变化及其与死亡时间(postmortem interval,PMI)的关系.方法放血处死家猪后取带眼睑的眼球96只,随机分为24组,在避光、温度(15±2)℃、湿度(50±5)%条件下分别放置2～96h,采集玻璃体液,运用全自动生化检测仪、超高效液相色谱检测K+、Na+、Cl-及次黄嘌呤(hypoxan-thine,Hx)浓度:用SPSS统计软件检测数据进行统计分析.结果玻璃体液中K+、Hx的浓度与PMI呈线性相关,R2分别为0.767和0.793;K+和Hx两者浓度与PMI经二元线性回归分析,R2为0.866;玻璃体液中Na+、Cl-浓度与PMI无明显相关性.结论 玻璃体液K+、Hx浓度变化可作为较为客观的推断PMI的参考指标.两者与PMI的二元回归方程推断死亡时间有更高的准确性.