Objective To compare the expression of tumor necrosis factor-α (TNF-α) between brain and peripheral organs after cerebral contusion in order to provide the scientific theoretical basis for forensic pathological diagnosis and wound age estimation. Methods Brain and peripheral organs including heart, liver, spleen, lung, kidney tissues of 45 SD rats after the cerebral contusion were obtained and TNF-α of these tissues were analyzed with immunohistochemistry methods. Results TNF-α was detected at 1 h in brain, reaching maximum at 6 h and 3 d after the cerebral contusion, and then decreased but still kept at high expression level at 7 d. TNF-α was detected at 1 h after the cerebral contusion in heart, liver, spleen, lung and kidney tissues. The number of cells expressing TNF-α increased gradually, reaching maximum at 3 d after the contusion of brain, and then decreased but still kept at high expression level at 7 d. Conclusion Besides the change of cerebral contusion, this study considered both the brain and peripheral organs. It is helpful for forensic pathological diagnosis and wound age estimation after contusion of brain.%目的 比较大鼠脑挫伤组织及其他器官组织中肿瘤坏死因子α(tumor necrosis factor-α,TNF-α)表达的差异,期望为法医病理学脑挫伤的诊断以及损伤时间的判断提供理论依据.方法 对45例SD大鼠脑挫伤后脑组织及心、肝、脾、肺、肾等器官组织进行TNF-α免疫组织化学染色,观察脑挫伤对不同器官中TNF-α表达水平的影响. 结果 脑损伤后1h,TNF-α在脑组织即有表达,分别在6h及3d出现两次高峰,7d表达减弱,但仍明显高于正常.心、肝、脾、肺、肾组织中TNF-α在颅脑损伤后1h也有表达,随损伤时间延长,阳性细胞逐渐增多,3d最高,7d虽有所下降,但仍显著高于正常. 结论 在研究脑损伤局部变化的同时,也可研究其他器官组织的相应变化,有助于脑损伤诊断及损伤时间推断.
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