大鼠脑损伤后EPO及其受体表达与损伤时间的关系

摘要

目的：研究大鼠脑损伤后脑组织中促红细胞生成素（erythropoietin，EPO）及其受体（erythropoietin receptor，EPOR）的表达与损伤时间的关系。方法72只SD大鼠随机分为对照组（36只）、脑损伤组（36只），在建模后1、2、4、8、12、24 h每组各取6只大鼠处死，取挫伤部位脑组织，应用实时荧光定量PCR和Western印迹法检测不同时间点EPO以及EPOR的mRNA表达和蛋白表达情况。结果伤后24 h内，EPO及EPOR的表达随损伤时间的增加而呈上升趋势。EPO的mRNA及蛋白表达与损伤时间之间存在相关性，其相关系数分别为0.875、0.911（P<0.05）；EPOR的mRNA及蛋白表达与损伤时间之间存在相关性，其相关系数分别为0.936、0.905（P<0.05）。结论大鼠颅脑损伤早期EPO及EPOR的表达呈逐步上升趋势，与损伤时间具有相关性，可以作为脑损伤时间推断的参考。%Objective To explore the relationship betw een injury age and expressions of erythropoietin (EPO ) and its receptor EPO R in the brain tissue of rats after cerebral injury. Methods Seventy-tw o rats w ere random ly divided into control group (36 rats) and cerebral injury group (36 rats). The rats w ere sac-rificed at 1, 2, 4, 8, 12, 24 h after cerebral injury (6 rats at each tim e point) and the brain tissues w ere extracted. The expressions of m RNA and protein of EPO and EPO R at different tim e points w ere de-tected by real-tim e fluorescent quantitative PC R and W estern bloting. Results The expressions of EPO and EPO R increased w ithin 24 h after injury. The expressions of m RNA and protein of EPO w ere relat-ed to the injury age, and the correlations w ere 0.875, 0.911, respectively (P<0.05). The expressions of m RNA and protein of EPO R w ere related to the injury age, and the correlation coefficients w ere 0.936, 0.905, respectively (P<0.05). Conclusion The expressions of EPO and EPO R increase gradually in the early stage of the rat’s cerebral injury, w hich are associated w ith the injury age and could be a useful value for estim ating injury age.