Objective T o detect diatom in the organs of drow ners by enzym e com bined w ith strong acid digestion m ethod, and evaluate its application value. Methods A total of 40 cases w hich have been identified as drow ning in local region w ere collected. Sam ples of the lung, liver, kidney, and the w ater of the scene w ere also gathered from each case. Strong acid digestion m ethod, enzym e com bined w ith strong acid digestion m ethod, and enzym ic digestion m ethod w ere respectively perform ed to detect the diatom in the sam ples. T he com parative analysis w as m ade on digestion tim e, digestive pow er and de-tection rate of diatom , etc. Results E nzym e com bined w ith strong acid digestion m ethod w as significant-ly better than enzym ic digestion m ethod on digestion tim e and digestive pow er;enzym e com bined w ith strong acid digestion m ethod w ere obviously superior to strong acid digestion m ethod on the detection rate of diatom . Conclusion E nzym e com bined w ith strong acid digestion m ethod com bines the advan-tages of strong acid digestion m ethod and enzym ic digestion m ethod. It has the characters of operation safety w ith little pollution to environm ent, w hich is w orthy of further popularization and practice.%目的 采用酶消化联合强酸消化法对溺死尸体的器官进行硅藻检验,并评价其应用价值.方法 收集40例本地区确证为溺死的尸体,每例提取肺、肝、肾组织及现场水样,分别采用强酸消化法、酶消化联合强酸消化法、酶消化法对组织样本进行硅藻检验,从消化时间、消化能力、硅藻检出率等方面进行比较分析.结果 在消化时间、消化能力方面,酶消化联合强酸消化法明显优于酶消化法;在硅藻检出率方面,酶消化联合强酸消化法明显优于强酸消化法.结论 酶消化联合强酸消化法结合了强酸消化法和酶消化法的优点,并且操作安全,环境污染小,具有一定的推广和实践价值.
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