硫化氢中毒案件中血液硫离子的测定

摘要

Objective T o establish a gas chrom atography-m ass spectrom etry (G C-M S ) m ethod for the determ ination of sulfide ion in blood and apply it to the practical cases. Methods T he 1, 3, 5-tribro-m obenzene w as selected as an internal standard, and 0.2 m L blood sam ple w as collected and analyzed using G C-M S after α-B rom o-2, 3, 4, 5, 6-pentafluorobenzyl brom ide derivatization. Results T he m ass concentration of sulfide ion in blood had good linearity in the range of 0.2-40μg/m L w ith a lim it of detection (L O D ) of 0.05μg/m L . T he m ass concentration of sulfide ion w as less than 0.05μg/m L in blank blood from different sources such as healthy subjects and dead cases. In 3 sulfide poisoning cases, sul-fide ion w as detected in the blood sam ples of 6 victim s, and the m ass concentration range w as 1.02-3.13μg/m L . Conclusion T his study establishes a m ethod for investigation of sulfide ion in blood w hich has been applied successfully to the cases of fatal sulfide poisonings.%目的 建立血液中硫离子的气相色谱-质谱(GC-MS)测定方法,并将其应用于实际案例.方法 取血液0.2 mL,以1,3,5-三溴苯为内标,经五氟苄基溴衍生化后采用GC-MS分析.结果 血液中的硫离子在0.2~40μg/mL质量浓度范围内线性良好,最低检出限为0.05μg/mL.健康人群、死后等不同来源的空白血液中硫离子质量浓度不高于0.05μg/mL.3例硫化氢中毒案件中,6名死者血液中均检出硫离子,质量浓度在1.02~3.13μg/mL.结论 本研究建立了血液中硫离子测定方法,并成功应用于硫化氢中毒死亡案件.