Objective T o investigate the tim e-dependent expression of m etallothionein (M T ) 1A m R N A and M T 2A m R N A in contused skeletal m uscle of rats. Methods A total of 54 Sprague-D aw ley rats w ere used in this study. T he rats w ere divided into tw o parts:control group (n=6) and contusion groups (0.5, 1, 6, 12, 18, 24, 30, and 36 h after contusion, n=6). T otal R N A w as extracted from skeletal m uscle. T he expression levels of M T 1A m R N A and M T 2A m R N A w ere detected by SY B R G reen I real-tim e PC R . Results T he expression trends of the tw o potential m arker genes w ere related to w ound age. In ad-dition to 0.5 h, there w ere significant contrasts betw een the control group and contused group (P<0.05), about the expression levels of M T 1A m R N A and M T 2A m R N A in different phases. A s the extension of w ound age, the relative expression of M T 1A m R N A and M T 2A m R N A at 1 h, 6 h, 12 h and 18 h after contusion dem onstrated upgrade tendency until its expression levels in 18 h peak w ith 239.41±15.20 and 717.42 ±50.76, respectively. W hen tim e extends to 24 h after injury, the expression of above tw o m arks decreased, respectively. T he M T 1A m R N A and M T 2A m R N A expression levels increased at 30 h and then decreased. Conclusion D eterm ination of M T 1A m R N A and M T 2A m R N A levels by real-tim e PC R m ay be useful for the estim ation of w ound age.%目的 研究大鼠肌肉挫伤后金属硫蛋白(metallothionein,MT)1A mRNA、MT2A mRNA的时序性表达.方法 54只SD大鼠被分成挫伤组(挫伤后0.5、1、6、12、18、24、30和36 h组各6只)和对照组(6只).所有总RNA均取自大鼠的骨骼肌.利用SYBR GreenⅠ法进行RT-qPCR检测大鼠挫伤骨骼肌中MT1A mRNA、MT2A mRNA的相对表达量.结果 MT1A mRNA、MT2A mRNA在损伤后的变化趋势与损伤时间具有一定的相关性.除了挫伤后0.5 h组外,其余各组MT1A mRNA、MT2A mRNA的表达量与对照组相比差异均有统计学意义(P<0.05).损伤后1 h、6 h、12 h、18 h MT1A mRNA和MT2A mRNA的相对表达量呈现逐渐上升的趋势,在18 h时达到了峰值,分别为对照组的(239.41±15.20)倍和(717.42±50.76)倍;损伤后24 h时两者的表达量均明显减少;损伤后30 h再次上升,随后下降.结论 MT1A mRNA、MT2A mRNA可能对损伤时间推断有一定的意义.
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