中草药提取物抑制H2O2诱导人皮肤细胞凋亡的初步研究

摘要

建立了过氧化氢(H2O2)诱导人皮肤成纤维细胞损伤及凋亡模型,并研究几种具有抗氧化作用的复方中草药提取物对细胞损伤及凋亡的影响.采用MTT、Hoechst染色及TUNEL等方法检测过氧化氢诱导细胞后以及加入中草药提取物后细胞的存活率、细胞形态以及细胞凋亡的状况.实验结果显示:在浓度为0.8 mmol/L,作用时间6 h,过氧化氢能诱导人皮肤成纤维细胞损伤和凋亡(p＜0.05);而抗氧化类中草药复方提取物等物质能够显著提高过氧化氢诱导的细胞存活率的降低,并能明显减少凋亡细胞的数量(P＜0.05),表明其对过氧化氢导致的细胞损伤和凋亡作用具有一定的抑制和防护作用.%This study aims to establish the cell injury and apoptosis model of human dermal fibroblasts induced by hydrogen peroxide in vitro and investigate the protective effects of several Chinese herbs on hydrogen peroxide-induced oxidative stress injury. The viability of human dermal fibroblasts was measured with MTT chromatometry,then the morphological changes of cells were observed through Hoechst 33342 staining and the apoptotic cells were detected by TUNEL assay.The results showed that normal human dermal fibroblasts were damaged by hydrogen peroxide at a appropriate concentration (0.8 mmol/1) for 6 h. The results also indicated that Chinese herbs increased obviously the cell viability and decreased the apoptosis rate. It was concluded that Chinese herbs could protect human dermal fibroblasts from hydrogen peroxide-induced oxidative stress injury.