石榴皮多酚体外抗脂质过氧化作用研究

摘要

为研究石榴皮多酚的抗脂质过氧化作用及起关键作用的成分,采用3个脂质过氧化研究体系,即卵黄体系、低密度脂蛋白体系和大鼠肝脏匀浆体系,利用硫代巴比妥酸法测定了石榴皮多酚纯化物、安石榴苷标准品和鞣花酸标准品对体外金属离子诱导性脂质过氧化的抑制作用.结果表明:安石榴苷,鞣花酸和石榴皮多酚纯化物均能有效抑制体外金属离子诱导的脂质过氧化,并且具有良好的剂量效应关系,其抗脂质过氧化的能力强弱顺序依次是:安石榴苷＞鞣花酸＞石榴皮多酚纯化物＞茶多酚,说明安石榴苷是石榴皮多酚中抗脂质过氧化的的关键物质.%The purpose of this manuscript is to study the inhibitory effect of the pomegranate peel polyphenols on the peoxidation of poly unsaturated fatty acid and to identify the key composition. For this, three classical anti-lipid peroxidation model, namely yolk system, low density lipoprotein system and rat liver homogenate system were used to investigate the effect of the purified pomegranate peel polyphenols (PPPs), Punicalagin and ellagic acid (EA)on the antioxidant activity . Results showed that PPPs exhibited a good protection against metal ions - induced lipid peroxidation in vitro in a dose-depend s. In addition ,the trend in anti- lipid peroxidation activity was Punicalagin>EA>PPPs, from this we can concluded that Punicalagin the key anti -lipid peroxidation component in pomegranate peel polyphenol mixtures.