板栗多糖的分离纯化、结构分析及抗疲劳作用的研究

摘要

作者对板栗多糖进行了分离纯化和结构分析,并对其抗疲劳作用进行了研究.气相色谱分析结果显示板栗多糖CPS-a的单糖组分及其摩尔比为葡萄糖∶甘露糖∶木糖∶阿拉伯糖=7.1∶6.2∶5.1∶4.1,CPS-b的单糖组分及摩尔比为葡萄糖∶果糖∶甘露糖∶木糖∶阿拉伯糖=7.1∶6.4∶6.2∶5.0∶4.1.红外光谱分析结果显示CPS-a、CPS-b都含有吡喃环,CPS-a可能是呋喃糖衍生物,CPS-b的糖链中同时存在α-、β-糖苷键,可能是硫酸化多糖.板栗多糖抗疲劳测定结果显示:3个剂量的板栗多糖均能显著提高运动耐力,降低运动后血乳酸和血尿素氮的含量,提高肌糖原和肝糖原的含量.说明板栗多糖具有良好的抗疲劳作用,其最佳用量为200 mg/(kg·d),高剂量并无明显优势.%In this study,the isolation,purification and structural analysis of Chestnut Polysaccharides (CPS) were performed,and then the anti-fatigue effects were investigated.Gas chromatography of CPS-X indicates that the molar ratio of monosaccharide constituents of CPS-a is glucose∶mannose∶xylose∶arabinose=7.1∶6.2∶5.1∶4.1,and for CPS-b which is glucose∶fructose∶mannose∶xylose ∶arabinose =7.1∶6.4∶6.2∶5.0∶4.1.Infrared spectroscopy analysis shows that both CPS-a and CPS-b have the pyranoid ring,and CPS-a may be a furanose derivative,furthermore,the sugar chain of CPS-b,which may be a sulfated polysaccharide,contains both α-and [β-glycosidic bond.The anti-fatigue effects of CPS show that CPS in three different doses all significantly increase exercise tolerance,decrease the content of serum BUN and LAC and increase the content of muscle glycogen and hepatic glycogen after swimming.Thus,we conclude that CPS have an excellent effect of anti-fatigue and the optimum does is 200mg/kg.d,and the high doeses shows no superiority.