洋甘菊抑制酪氨酸酶活性成分研究

摘要

研究了罗马洋甘菊乙醇提取物对蘑菇酪氨酸酶活性的影响并对有效成分进行分离纯化.实验中以L-酪氨酸为底物,空白对照,采用比色法测定了罗马洋甘菊乙醇提取物不同乙醇浓度的洗脱物对蘑菇酪氨酸酶的抑制作用.结果表明:MCI30％(体积分数)乙醇浓度洗脱物对酪氨酸酶活性的抑制率最灵敏.该部分洗脱物经MCI、ODS-A柱分离,得到了3个单体化合物:山奈酚-3-O-β-D-吡喃葡萄糖苷、异鼠李素-3-O-β-D-吡喃葡萄糖苷、山奈酚-3-O-(6"-3-羟基-3-甲基-戊二酰基)-β-D-吡喃葡萄糖苷.%To investigate whether the extract with ethanol from Anthemis nobilis L.could be ascribed to the inhibition of tyrosinase and know about the active ingredients,the experiment was conducted.The inhibition rates of different concentration of ethanol elution on mushroom tyrosinase were tested by the colorimetric method using L-tyrosine as substrate.It finds out that the 30％ volume concentration of ethanol elution content is the most sensitive part on tyrosinase activity inhibition.The further result shows that the active part include kaempferol-3-O-β-D-glucopyranoside,isorhamnetin-3-O-γ-D-glucopyranoside,kaempferol-3-O-(6"-O-methyl)-γ-D-glucopyranoside.