鸡肉中弯曲菌定量检测方法的建立与初步应用

摘要

目的：参照国标方法,建立鸡肉中弯曲菌定量检测的CCDA平板计数法。方法通过对选择性CCDA培养基中添加不同浓度组合的抗生素和生长促进剂,确定最佳添加浓度组合,并与普通 CCDA 平板计数法进行比较,建立鸡肉中弯曲菌定量检测方法,同时评价该方法的特异性和检测限。结果添加一定浓度抗生素和生长促进剂的选择性CCDA培养基和普通CCDA培养基对弯曲菌标准菌株定量计数的吻合度为(100±5)%；除弯曲菌外,其他5种肠道细菌在该CCDA培养基中均不能生长；该方法对弯曲菌菌悬液可检测至10 CFU/mL。运用该方法对50份鸡肉样品进行调查研究,共检测出22份阳性样品,弯曲菌检出率为44%,平均检测值为20 CFU/100 cm2,检测结果与国标方法阳性符合率为100%。结论该方法简便、特异性好、准确度高,为定量检测鸡肉中弯曲菌提供了新技术。%ABSTRACT:Objective A quantitative detection method was developed to detect Campylobacter spp. con-tamination in chicken meat. Methods According to the methods of national standards, a kind of selective CCDA medium which contains antibiotics and growth promoter was selected and modified to establish a quan-titative method for the detection of Campylobacter spp.. Results The conformity of the numbers of Campy-lobacter in selective CCDA medium and conventional CCDA medium was (100±5)%. The specific results showed that no other bacteria strains could be cultured in this selective CCDA medium except Campylobacter;the limit of detection was as few as 10 CFU/mL sample. In this way, 50 chicken meat samples were investi-gated for Campylobacter. The accuracy of this method was shown to be 100%when compared to GB/T 4789. 9-2008. Conclusion The method was simply operated with high accuracy which was proved to be practical for detection of Campylobacter spp..