黑褐新糠虾血细胞形态及其与日本新糠虾对溶藻弧菌敏感性的比较

摘要

In consideration of the differences between Neomysis awatschensis and Neomysis japonica in the growth, reproduction, susceptibility and other aspects, the susceptibility of two Neomysis to Vibrio alginolyticus was studied, which could help to explore to the root of the phenomenon in immunology. Two staining methods of Giemsa and wright' s were used in this experiment of the haemocytes of the adult N. Awatschensis, and haemocytes of the mysis were classified base on the large/small size, the presence/absence of cytoplasmic granules,the number of granules,and nucleus to cytoplasm(N/C)ratio and the proportion of various haemocytes were also carried out. The results of these studies showed: three types of haemocytes were identified in N. Awatschensis-hyaline cells, semigranular cells, granular cells, ascending in the size and declining in the N/C ratio,successively. To further check the statistics of the rate of three types haemocytes, the observations indicated that the highest rate of granular cells was about(48.38% ±0.05% ) ,the lowest of hyaline cells about( 16.40% ±0.01% ) ,the moderate rate of semigranular cells about(35.22% ±0.04% ). By contrasting the dyeing time and dyeing effect of the two staining methods, Wright' s staining was more appropriate for the mysis haemocytes. When exposured in aquaculture water containing various concentration of V. Alginolyticus,N. Awatschensis began to die at 72 h while N. Japonica at 48 h,and had a higher death rate than that of N. Japonica in all the concentrations. Compared with N. Japonica, N. Awatschensis was less susceptible to V. Alginolyticus, and the reason of such difference could be associated with the higher ratio of granular cells (including semigranular and granular cells) in N. Awatschensis.%实验采用Giemsa与Wright' s两种染色方法对黑褐新糠虾血细胞进行染色,根据血细胞大小,所含颗粒与否,颗粒多少和核质比将其分型,并对各型血细胞所占比例进行统计.结果表明,通过比较Giemsa染色与Wright's染色两种方法的染色时间和效果,Wright's染色更加适合糠虾血细胞.通过染色,将黑褐新糠虾血细胞分为3种类型,它们分别为透明细胞、半颗粒细胞和颗粒细胞.它们的胞体大小依次增大,核质比依次降低.对此3种类型血细胞比例分别统计后发现,颗粒细胞所占比例最多约( 48.38％±0.05％),其次是半颗粒细胞约为(35.22％±0.04％),最少的为透明细胞约为(16.40％±0.01％).对不同浓度溶藻弧菌的感染试验发现,日本新糠虾在较短的时间内出现死亡,即48h,而黑褐新糠虾72 h出现死亡,各浓度组中日本新糠虾死亡率均高于黑褐新糠虾.黑褐新糠虾相对日本新糠虾来说,对溶藻弧菌具有较低的敏感性,可能与其血细胞中颗粒和半颗粒比例较高有关.