无论在纯化标记方面还是生物功能方面,造血干细胞都是目前研究最深入的成体干细胞,具有很大的临床应用价值.但是由于造血干细胞以极低比例存在于小鼠骨髓中,这给其分离纯化及功能研究带来了一定的难度.利用细胞表型和功能染料,通过免疫磁珠和流式细胞术可分离纯化骨髓和外周血中的造血干细胞.40年来,已有很多表面免疫表型确定可以富集小鼠造血干细胞,并且不断有新的免疫表型被发现,CD34- c-Kit+ Sca-1+ lineage marker-(CD34 - LSK)是最常用的小鼠造血干细胞表型.本文就小鼠造血干细胞表型及分离纯化的研究做一综述.%Hematopoietic stem cells(HSC), the well-characterized adult stem cells both in the markers and function, show tremendous therapeutic potential. However, the level of hematopoietic stem cells in bone marrow is very low, which makes it difficult to work with. Based on cell surface markers and dye staining, HSC can be isolated from bone marrow and peripheral blood by using magnetic-activated cell separation and fluorescence-activated cell sorting. Over 40 years of research, many surface markers have been identified to purify mouse HSC, and CD34 ~ LSK cells are regarded as the mostly used enrichment of HSC in mouse bone marrow. The purpose of this review is to provide insight into the advance of these markers, and isolation and purification of HSC.
展开▼
