噬血细胞性淋巴组织细胞增多症患者血清中新蝶呤的检测及意义

摘要

本研究通过检测噬血细胞性淋巴组织细胞增多症(HLH)患者外周血血清中新蝶呤(Npt)水平,探讨其在HLH中的意义.应用酶联免疫吸附法(ELISA)分别检测20例HLH患者治疗前后和15例正常对照者外周血血清Npt水平及sCD25水平.结果显示,HLH组诊断时血清Npt和sCD25水平分别较正常对照组明显升高(P＜0.0001).HLH组患者治疗后血清Npt和sCD25水平分别较治疗前明显下降(P＜0.0001).将治疗前后Npt水平与sCD25水平分别进行相关性分析,发现两者具有显著相关性(治疗前r=0.81,P＜0.05；治疗后r=0.65,P＜0.05)；与此同时,治疗前Npt水平与血清铁蛋白亦有显著相关性(r=0.55,P＜0.05).结论:血清Npt可能在HLH的发病机制中起着重要作用,其水平的升高对疾病的早期诊断及病情评估具有重要意义.%This study was aimed to detect the peripheral blood serum neopterin ( Npt) level in the patients with hemophagocytic lymphohistocytosis (HLH) and to explore its significance in HLH. The enzyme-linked immunosorbent assay (ELISA) was applied to detect the serum Npt level and sCD25 level in 20 HLH patients before and after treatment and 15 healthy controls. The results indicated that the serum Npt and sCD25 levels in HLH patients were significantly higher than those in healthy controls (P < 0. 0001). The serum Npt and sCD25 levels in the HLH group decreased significantly after treatment, respectively (P <0.0001). The correlation analysis of Npt with sCD25 before and after treatment showed that they had significant correlation ( r = 0. 81, P < 0. 05 before treatment; r = 0. 65, P < 0. 05 after treatment). Meanwhile, the level of serum Npt and ferritin had a significant correlation in newly diagnosed HLH patients (r = 0. 55,P <0. 05). It is concluded that the serum Npt may play an important role in the HLH pathogenesis, the enhancement of Npt levels has an important significance for diagnosis and evaluation for HLH.