P53-inducible Gene 3(PIG-3)在弥漫性大B细胞淋巴瘤中的表达及意义

摘要

本研究旨在探究P53-inducible gene3(PIG-3)在弥漫性大B细胞淋巴瘤(DLBCL)中的表达情况及其与淋巴瘤发病机制的相关性.应用免疫印迹(Western blot)和RT-PCR等方法,检测弥漫性大B细胞淋巴瘤患者和健康成年人PIG-3蛋白的表达情况,并判断其与淋巴瘤发病机制的相关性.结果表明,Western blot检测弥漫性大B细胞淋巴瘤细胞中PIG-3蛋白表达明显低于对照组,化疗后6个月PIG-3蛋白表达较化疗前升高.RT-PCR结果显示,扩增产物大小为1285 bp,与理论值吻合.结论:PIG-3表达下调可能与弥漫性大B细胞淋巴瘤发生密切相关,故PIG-3有可能作为弥漫性大B细胞淋巴瘤治疗及预后检测的一个重要指标.%This study was aimed to investigate the expression of p53-inducible gene 3(PIG-3) in diffuse large B cell lymphoma( DLBCL) and the relationship between PIG-3 and the pathogenesis of lymphoma. The expression of PIG-3 in 20 patients with DLBCL and 20 healthy adults( as a control group) was detected by Western blot and RT-PCR. The results showed that the expression of PIG-3 protein in patients with DLBCL was significantly lower than that in controls, but the expression of PIG-3 was higher after chemotherapy for 6 monthes than that before chemotherapy. RT-PCR detection demonstrated that the size of PIGS amplified product is 1285 bp, which is coincident with theoretic value. It is concluded that the down-regulation of PIG-3 expression may be closly related to pathogenesis of DLBCL, so the PIG-3 gene can considered as a important marker for judging therapeutic efficancy and prognosis of patients with DLBCL.