高三尖杉酯碱对K562细胞NF-κB和BCL-2蛋白表达的影响

摘要

This study was aimed to investigate the effect of homoharringtonine (HHT) on K562 cell proliferation, apoptosis and expression of BCL-2 and NF-kB proteins. The cells proliferation was assayed with MTT method, the cell apoptosis, cell cycle and BCL-2 expression were analyzed with flow cytometry, NF-kB protein expression was detected with Western blot. The results showed that HHT concentration-denpendently inhibited proliferation of K562 cells, the IC50 at 48 h was 43. 89 ng/ml. Treated with HHT 10 ng/ml for 48 h, K562 cell apoptosis significantly increased, cell cycle was blocked at G0/G1, the expression level of BCL-2 and NF-kB proteins was lower than that in control group(P < 0.05) . It is concluded that HHT may inhibit the proliferation of K562 cells, and down-regulating expression levels of BCL-2 and NF-kB may be one of its anti-CML mechanisms.%本研究旨在探讨高三尖杉酯碱(HHT)对K562细胞增殖、凋亡及BCL-2和NF-κB蛋白表达的影响.不同浓度HHT作用K562细胞后用MTT法、流式细胞仪、Western blot等方法分别检测细胞增殖、凋亡、BCL-2及NF-κB蛋白表达水平.结果表明,HHT作用48 h,浓度依赖性抑制K562细胞增殖,IC50为43.89 ng/ml.HHT 10 ng/ml作用48 h,K562细胞凋亡率明显增高；细胞周期阻滞于G0/G1期；BCL-2和NF-κB蛋白表达水平明显低于对照组(P＜0.05).结论:HHT对K562细胞有显著的增殖抑制、细胞周期阻滞和凋亡诱导作用,抑制NF-κB和BCL-2表达可能是其抗CML的机制之一.