Objective:To study the expression of stromal cell derived factor-1α (SDF-1α) receptor CXCR7 in acute monocytic leukemia (AML-M5),and its effects on proliferation,apoptosis,invasion of acute monocytic leukemia cell line THP-1.Methods:CXCR7 protein and mRNA expression levels in THP-1 cells and peripheral blood mononuclear cells (PBMNC) from the newly diagnosed AML-M5 patients and normal individuals were detected by flow cytometry,Western blot and RT-PCR respectively.CCK8,Annexin V/PI double staining and Transwell assay were used to observe the effects of CXCR7 on the proliferation,apoptosis,and invasion of THP-1 cells in vitro.Results:The expression of CXCR7 on immature cell surface of the newly diagnosed AML-M5 patients was significantly higher than that in the control group (P ＜ 0.05).CXCR7 was also highly expressed on THP-1 cells surface.The CXCR7 protein and mRNA levels in THP-1 cells and PBMNC of AML-M5 patients were significantly higher than those in the control group (P ＜ 0.05).The THP-1 cell proliferation activity was higher in SDF-1α-treated group,but this activity could be inhibited by CXCR7 antibody (P ＜ 0.01).CXCR7 antibody did not affect THP-1 cell apoptosis (P ＞ 0.05).CXCR7 antibody could inhibit SDF-1oα-induced TAP-1 cell invasiveness (P ＜ 0.01).Conclusion:CXCR7 highly expresses in AML-M5 patients and THP-1 cells,and involves in cell proliferation and invasion.The blocking CXCR7 expression can reduce the risk of AML-M5 cell infiltration.%目的:探讨基质细胞衍生因子-1α(SDF-1α)受体CXCR7在急性单核细胞白血病(AML-M5)中的表达,及其对急性单核细胞白血病细胞系THP-1增殖、凋亡和侵袭能力的影响.方法:应用流式细胞术、Western blot、RT-PCR分别检测初诊AML-M5患者和正常人外周血单个核细胞(PBMNC)及THP-1细胞CXCR7蛋白和mRNA表达.CCK8法、Annexin V/PI标记法和Transwell法观察CXCR7对THP-1细胞体外增殖、凋亡和侵袭的作用.结果:在初诊AML-M5患者PBMNC幼稚细胞表面CXCR7表达高于正常对照(P＜0.05),在THP-1细胞表面CXCR7也有高表达;THP-1细胞和AML-M5患者PBMNC中CXCR7蛋白和mRNA水平均明显高于正常对照(P ＜0.05);SDF-1α刺激可增高THP-1细胞增殖活性,但这种增殖活性可被CXCR7抗体抑制(P ＜0.01);CXCR7抗体不影响THP-1细胞凋亡(P＞0.05);CXCR7抗体可明显抑制SDF-1α诱导的THP-1细胞侵袭能力(P＜0.01).结论:CXCR7在急性单核细胞白血病患者及THP-1细胞中均有高表达,并参与细胞增殖和侵袭,阻断CXCR7表达有可能降低急性单核细胞白血病的浸润风险.
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