急性白血病IL-32水平动态变化与病情发展的关系

摘要

Objective:To investigate the correlation between dynamic change of IL-32 level and disease development in the patients with acute leukemia(AL) and to explore its clinical significance.Methods:The serum IL-32 levels and IL-32 mRNA expression in 82 cases of AL and 30 healthy persons were measured by ELISA and real-time PCR.Results:Compared with healthy persons,the serum IL-32 protein level and IL-32 mRNA expression in AL,acute lymphoblastic leukemia(ALL) and acute non-lymphocytic leukemia(ANLL) groups all were significantly higher(P ＜ 0.05).The serum level of IL-32 protein and mRNA expression in newly diagnosed,PR and relapsed ALL and ANLL groups were all higher than those in the control group(P ＜ 0.05),and the serum protein level of IL-32 in relapsed ALL and ANLL groups were higher than that in other stage group(P ＜0.05).The serum levels of IL-32 protein and mRNA were not significantly different between CR and control group (P ＞ 0.05).Conclusion:The IL-32 in the peripheral blood of patients with AL has been found to be closely related with the occurrence and development of disease,therefore,monitoring the dynamic changes of serum IL-32 level would contribute to the clinical judgment of the severity,the IL-32 levels can be used as indicators for the therapeutic efficacy for AL.%目的:检测急性白血病(AL)患者的白介素32(IL-32)的表达,探讨其表达的临床意义.方法:应用酶联免疫吸附法和实时荧光定量PCR分别检测82例AL患者和30例健康人血清中IL-32的浓度及其mRNA的表达.结果:与健康人群相比,AL组及其两个分型组(ANLL和ALL)的外周血清IL-32蛋白水平及IL-32 mRNA表达水平均显著提高(P＜0.05).ANLL和ALL处于初诊、PR和复发期的患者的IL-32蛋白水平及IL-32 mRNA表达水平明显高于健康人群(P＜0.05),并且其复发期组患者的IL-32蛋白水平及IL-32 mRNA表达水平显著高于其他分期组患者(P＜0.05);而在ANLL和ALL患者中处于CR期患者的IL-32蛋白水平及IL-32 mRNA表达水平与健康人群相比无显著性差异(P＞0.05).结论:急性白血病患者外周血中IL-32与其自身的病情发展有密切联系,检测患者体内IL-32的动态变化,有助于了解疾病的进展,因而IL-32水平可作为监测急性白血病的疗效指标.