DMSO促进人胚胎干细胞体外造血分化

摘要

目的:探讨DMSO在人胚胎干细胞造血分化中的作用.方法:利用已建立的人胚胎干细胞逐级诱导分化体系,通过流式细胞术分析并结合免疫荧光测定研究DMSO在造血分化中的作用,同时通过在不同时间点添加DMSO,确定其发挥作用的窗口期.结果:免疫荧光及流式细胞术分析发现,在人胚胎干细胞造血分化过程中添加DMSO显著促进CD43+造血祖细胞的产生;流式细胞术分析发现,DMSO显著促进APLNR+侧板中胚层细胞和CD31+ CD34+生血内皮细胞的产生;在侧板中胚层细胞产生的窗口添加DMSO显著促进CD31+ CD34+生血内皮细胞和CD43+造血祖细胞的产生.结论:DMSO通过促进侧板中胚层的产生促进人胚胎干细胞造血分化能力,在侧板中胚层发生窗口添加DMSO能够显著促进造血祖细胞的产生.%Objective:To explore the role of dimethyl sulfoxide (DMSO) in the hematopoietic differentiation of human embryonic stem cells (hESCs).Methods:The role of DMSO in hematopoietic differentiation of hESC was investigated by using a established stepwise hematopoietic differentiation system from hESC,immunofluorescouse assay and flow cytometry.Furthermore,the window phase of DMSO action was explored by adding it to the different stage of hematopoietic differentiation.Results:Immunofluorescence and flow cytometry analysis showed that DMSO significantly promoted the generation of CD43 + hematopoietic progenitor cells (HPC).The flow cytometry demonstrated that DMSO profoundly promoted the generation of APLNR + lateral plate mesoderm cells and CD31 + CD34 + hemogenic endothelium progenitors (HEP).The addition of DMSO in the window phase of lateral plate mesoderm cell generation could markedly improve the generation of hematopoietic progenitor cells.Conclusion:DMSO promotes hematopoietic differentiation of hESC through enhancing the generation of APLNR positive lateral plate mesoderm cells.The addition of DMSO in the window phase of lateral plate mesoderm cell generation can significantly improved the generation of hematopoietic progenitor cells.