雷公藤不同提取部位对小鼠肝肾的损伤作用

摘要

Objective:To observe the effect of the hepatic and kidney injury on mice after taking different extractions of tripterygium. Methods: After extracting different parts of tripterygium, administration of different extraction parts was conducted for 5 and 10 days respectively. Hepatotoxicity and nephrotoxicity related index was indected for finding out the more apparente part of hepatic and renal injury. Results:The alcohol,acetidin and n-butyl alcohol extracts of tripterygium can induce the content of ALT and AST in blood increasing after administration. After administration of 5 days,high dosage group of acetidin and n-butyl alcohol extracts and alcohol extracts group can induce the content of BUN increasing. After administration of 10 days,high dosage group of n-butyl alcohol extracts and alcohol extracts group can induce the content of BUN increasing. Alcohol extracts group can also induce the content of Scr increasing. Conclusion:The hepatotoxicity of tripterygium is stronger. Its toxic component concentrates on alcohol extracts, especially on acetidin and n -butyl alcohol extracts. While the nephrotoxicity of tripterygium is not obvious,and this effect is related with alcohol extracts of Tripterygium.%目的 观察雷公藤不同提取部位对小鼠肝肾毒性损伤的作用.方法 提取分离雷公藤不同部位,给小鼠分别灌服5、10 d,检测给药后小鼠体内的肝肾损伤相关功能指标,研究雷公藤的肝肾损伤较明最的部位.结果 给药5、10d后,雷公藤醇提物部位、乙酸乙酯部位和正丁醇部位可导致小鼠血中谷丙转氨酶和谷草转氨酶的升高;给药5d后,乙酸乙酯高剂量组和正丁醇高剂量组以及醇提物高、低剂量组的尿素氮(BUN)值升高,但是对血肌酐(Scr)影响不大；给药10d后,正丁醇高剂量组以及醇提物高、低剂量组的BUN升高,醇提物高、低剂量组Scr值升高.结论 雷公藤的肝毒性较强,且毒性成分集中在醇提物部位,而以乙酸乙酯部位和正丁醇部位较多.雷公藤不同部位短期给药产生的肾脏毒性不明显,对肾脏产生的影响主要和醇提物部位有关.