早期针刺对创伤性脑损伤大鼠血清GFAP和S-100B蛋白水平的影响

摘要

目的 观察针刺对创伤性脑损伤(TBI)大鼠血清胶质纤维酸性蛋白(GFAP)和S-100B蛋白水平的影响,评估针刺对创伤性脑损伤的疗效.方法 按随机数字表法随机将120只雄性SD大鼠随机分成3组,每组40只:假手术组、模型组、针刺组.假手术组只开骨窗,不进行模型制备;模型组和针刺组制备TBI模型:针刺组造模后即接受针刺治疗,每日1次.术后6h、1d、3d、5d4个时间点每组均随机选出10只大鼠进行血清GFAP、S-100B蛋白水平和改良的神经功能缺损(mNSS)评分观察与检测.结果 相较假手术组,模型组6h即检测到血清GFAP和S-100B蛋白水平升高(P<0.01).伤后6h、1d检测发现针刺组GFAP水平比同期模型组降低(P<0.05),伤后6h、1d、3d检测发现针刺组S-100B蛋白水平比同期模型组显著降低(P<0.01).4个检测时间点上,模型组大鼠mNSS评分均高于同期假手术组(P<0.01),针刺组评分显著低于同期模型组(P<0.05或P< 0.01).结论 TBI早期采用针刺干预可明显减轻大鼠脑损伤的程度.%Objective: To observe the effect of acupuncture on serum glial fibrillary acidic protein (GFAP) and S-100B protein in rats with traumatic brain injury (TBI),and to evaluate the therapeutic effect of acupuncture on traumatic brain injury.Methods: 120 male SD rats were randomly divided into 4 groups,40 rats in each group:the sham operation group, the model group and the acupuncture group.In the sham operation group, only the bone window was opened and the model was not prepared;the TB1 model was prepared in the model group and the acupuncture group;the acupuncture group received acupuncture treatment once a day after molding.6 h, 1 d, 3 d and 5 d after operation were taken as four time points.10 rats were randomly selected from each group at one time point.The contents of serum GFAP,S-100B protein and mNSS were observed and detected.Results: Compared with the sham operation group,the serum GFAP and S-100B protein levels increased in the model group only after 6 h(P<0.01).At 6h and 1d after injury,it was showed that the GFAP level in the acupuncture group was lower than that in the model group(P<0.05).Data of 6 h, 1 d and 3 d showed that the level of S-100B protein in the acupuncture group was significantly lower than that in the model group (P< 0.01).The mNSS scores of the model group were higher than those of the sham operation group (P< 0,01) and the score of the acupuncture group was significantly lower than that of the model group (P< 0.01 or P< 0.05).Conclusion: Early intervention with acupuncture can obviously relieve the brain damage in rats with TBI.