Chemical amendments were applied to and red ramie cultivated in multi-metal contaminated acid soil of Dabaoshan mining site, and then response of the microbial community therein in biological feature was investigated. The chemical amendments significantly improved activity of the soil microbial communities in plots before the cultivation of red ramie showing an order of manure + limestone ≈ fly ash > dolomite > limestone > control in effect. Treatment manure + limestone was the most significant in improving metabolic diversity of the microbial community. However, the order altered in the presence of red ramie into the one of manure + limestone > fly ash > limestone > control > dolomite. The effect of manure + limestone was more significant with red ramie than without the crop. In plots with red ramie, manure + limestone stimulated root exudation of L-serine, 4-hydroxy benzoic acid, L-arginine etc., enabling soil microbes to better use carbon sources of amine and amino acid types. Soil microbes in all the treatments were found to have been enhanced in ability to utilize saccharides, amino acids, amines and some others as carbon sources, which contributes to biological remediation of heavy metals contaminated soils.%应用BIOLOG技术对广东省大宝山矿山周边多金属污染土壤修复过程中微生物生态特征的变化进行分析.结果表明,种植红麻(Hibiscus cannabinus)前施用改良剂可以显著提高土壤微生物活性,各处理土壤微生物活性由大到小依次为有机肥+石灰石≈粉煤灰、白云石、石灰石和对照(未施用改良剂),其中施用有机肥+石灰石对提高多金属污染土壤微生物的代谢多样性有显著效果；种植红麻后各处理土壤微生物活性由大到小依次为有机肥+石灰石、粉煤灰、石灰石、对照和白云石,有机肥+石灰石和红麻联合修复对增加土壤微生物活性的效果更显著；种植红麻后,施用有机肥+石灰石可以刺激根系分泌L-丝氨酸、4-羟基苯甲酸和L-精氨酸等碳源,使微生物对胺类和氨基酸类碳源的利用能力大幅提高.各处理土壤微生物对糖类、氨基酸类和胺类等碳源的利用能力增强,有助于重金属污染土壤的生态修复.
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