建立了反相高效液相色谱同时测定西红花苷Ⅰ和西红花酸的方法.采用C8色谱柱,流动相为甲醇水冰醋酸溶液,甲醇、水和冰醋酸比例为75∶24.5∶0.5,流速为0.6mL.min-1,检测波长为440 nm,在该色谱条件下,西红花苷Ⅰ和西红花酸得到较好的分离,西红花苷Ⅰ和西红花酸浓度在0.625～ 40 μg·mL-范围内峰面积与进样量呈良好的线性关系,方法平均回收率为101.33％.该方法具有重复性好、准确、快速等优点,适用于西红花苷Ⅰ和西红花酸的同时测定.%In this study, we established a method for determination of crocin I and crocetin simultaneously by reverse phase High-Performance Liquid Chromatography. Elite C8 hromatographic column (4.6 mm ×250 mm, 5μm) was used, the mobile phase was methanol water acetic acid solution, their proportion of methanol, water and acetic acid were 75:24.5:0.5, the detected wavelength was 440 nm, and the flow speed was 0.6 mL·min-1 rn. The crocin I and crocetin got better separation, both the contents of crocin I and crocetin and their peak areas were in good linear relationships, respectively. The average recovery was 101. 33% . This method showed good repeatability , quantitative, accuracy and rapid, and could be used effectively for the simultaneous determination of crocin I and crocetin.
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