喜树内生真菌的分离及产10-羟基喜树碱菌的鉴定

摘要

Six endophytic fungi isolated from Camptotheca acuminate Decne were cultured in taper flask under shaking. The organic extracts of mycelium were analyzed with thin layer chroma-tography (TLC) and high performance liquid chromatography (HPLC). The results showed one of the strains produced the 10-hydroxycamptothecin (HCPT) with output of 410 f*g/L and was named XK002. Morphological observation indicates that XK002 belongs to Mycelia sterlia.%从喜树(Camptotheca acuminate Decne)树皮中分离、纯化得到6株内生真菌,经摇瓶培养后,采用TLC法与HPLC法对其菌丝体提取物进行分析,结果表明其中1株内生真菌(XK002)产10-羟基喜树碱(10-hydroxycamptotheein,HCPT),将此菌株命名为XK002,其10-羟基喜树碱产量为410/μg/L.对菌株XK002菌落、菌丝体和孢子的有无进行了研究,表明菌株XK002属于无孢菌群(Mycelia sterlia).