响应面法优化皖南山核桃壳棕色素的提取工艺

摘要

研究皖南地区山核桃壳中棕色素的最佳提取工艺。在单因素试验的基础上，以提取率为评价指标，运用Box-Behnken试验设计和响应面分析法确定了主要影响因素液料比、提取时间和提取温度的最佳条件。结果表明，棕色素的最佳提取工艺条件为：液料比16 mL·g-1，提取时间40 min，提取温度63℃。此条件下，棕色素提取率为4.2%，与理论预测值4.4%非常接近。因此，利用响应面分析方法对核桃壳棕色素提取工艺进行优化，可获得最优的工艺参数。%This paper aims at optimizing the extraction of brown pigments from hickory hulls in south Anhui province. On the basis of single factor experiments,the influence of liquid-solid ratio,extraction time and tem-perature on brown pigment extraction yield was determined according to Box-Behnken design and response sur-face methodology. Experiment results showed the optimum extraction conditions were as follows:the liquid-solid ratio of 16 mL·g-1 for an extraction time of 40 min at 63 ℃. Under these optimal conditions,the experimental value of brown pigment extraction yield was 0.042 g·g-1,in good agreement with the predictive value 0.044 g·g-1. Therefore,the parameters of brown pigment extraction process from hickory hulls can be optimized by response surface methodology.