大气压冷等离子体提高乙醇转化率的实验参数优化研究

摘要

Process conditions were investigated to improve ethanol yield in Saccharomyces cerevisiae by cold plasma at atmospheric pressure. According to single factor investigation, a mathematical model was established using Box-Behnken central composite experimental design, with treated time of plasma discharge, supply voltage, and treated volume of cell suspension as the impact factors and with ethanol yield as their response. This was followed by response surface analysis. It was found that the optimal process conditions for enhanced ethanol yield in Saccharomyces cerevisiae by plasma discharge were as follows： treated time 1 min, supply voltage 24 V, treated volume of cell suspension 9 mL, and that the resultant ethanol yield was 0.58 g/g with an increase of 23.6% compared to the control.%以酿酒酵母（Saccharomycescerevisiae）为研究对象，研究大气压冷等离子体激活酿酒酵母提高乙醇转化率的工艺条件。在单因素实验基础上，选取等离子体处理时间、等离子体电源电压和处理菌液体积为影响因子，以乙醇转化率为响应值，应用Box．Behnken中心组合实验建立数学模型，进行响应面分析。结果表明，大气压冷等离子体提高乙醇转化率的最佳实验参数为：处理时间1min，电源电压24V，处理菌液体积9mL。在此条件下，乙醇转化率达到O．58g／g，比未处理过的酿酒酵母发酵葡萄糖生成乙醇的转化率高23．6％。