对山西历山2尾野生大鲵Andrias davidianus mtDNA中Cyt b和ATPase 6基因的部分序列进行了检测,并与GenBank中收集的14尾外地样本进行比较,用Mega 5.0软件对305 bp Cyt b片段和371 bp ATPase 6片段进行分析.结果表明:Cyt b片段有10个不同的单倍型,共检测出14个变异位点,占核苷酸总数的4.58％,其中有12个简约信息位点;ATPase 6片段有13个不同的单倍型,检测出30个变异位点,占核苷酸总数的8.09％,其中有18个简约信息位点,Cyt b比ATPase 6更为保守,但两序列中碱基组成均表现为鸟嘌呤缺乏(＜18.9％),且多数变异发生在密码子第3位.合并序列构建的分子系统树表明,历山大鲵与陕西、四川及部分湖南个体聚为一支,历山大鲵与广西样本的遗传距离最远,与四川、陕西样本的遗传距离较近.%Partial sequences of Cyt b and ATPase 6 genes in mtDNA were sequenced in two wild individuals of Chi-nese giant salamander Andrias davidianus from Lishan, Shanxi and compared with 14 samples collected from other areas in China (from GenBank). The analysis of Mega 5.0 showed that there were 14 variable loci in the 305 bp Cyt b fragment, while there were 30 variable loci in the 371 bp ATPase 6 fragment. Nucleotide variation in the two sequences indicates that the Cyt b was found to be more conserved than ATPase 6. However, both sequences were shown to be lack guanine (<18.9%) in base composition and that the variations were mostly observed at the third site of the codon. The phylogenetic trees based on the combined sequences of Cyt b and ATPase 6 revealed that Lis-han population was clustered into a group with Shaanxi and Sichuan populations. Lishan giant salamander popula-tion is found to be special in the corresponding fragments of mtDNA, which is genetically different from Guangxi population and closer to Sichuan and Shaanxi populations.
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