光棘球海胆壳中化学成分的分离、鉴定及其抗肿瘤活性

摘要

Two compounds were isolated from shells of sea urchin Strongylocentrotus nudus by column chromatogra-phy on silica gel and thin layer chromatography and identified by spectroscopic analysis and physical-chemistry properties. The anti-tumor activity of the two compounds was evaluated by the MTT assay and SRB assay. The re-sults showed that the two compounds were identified as palmitate(compound 1) with unsaturated double bonds and glyceroyl monopalmitate ( compound 2 ) . In vitro trials revealed that the glyceroyl monopalmitate exhibited the po-tent inhibitory activities against five cancer cells, indicating that this compound is considered as potential anticancer candidate for further study.%采用硅胶柱色谱及薄层制备色谱等手段，从光棘球海胆Strongylocentrotus nudus壳中分离纯化出2种化合物，并对所获得的化合物经过理化性质、波谱分析和文献对照的方法进行结构鉴定，同时对所获得的化合物进行体外抗肿瘤活性测试。结果表明，从光棘球海胆壳中分离得到的2种化合物分别为棕榈酸(化合物1)和单棕榈酸甘油酯(化合物2)。体外抗肿瘤细胞活性测试表明，单棕榈酸甘油酯具有显著的抑制肿瘤细胞生长的活性，其可作为潜在的抗肿瘤药物进行进一步研究。