澳大利亚厚皮海绵化学成分分离鉴定及其抗肿瘤活性研究

摘要

Two compounds were isolated from sponge Craniella australiensis by column chromatography on silica gel and gel filtration chromatography, and identified by spectroscopic analysis and physical-chemistry property to eval-uate the application of the sponge in preparation of marine drugs. The inhibition on tumor cells of both extracts from the sponge was evaluated by CCK-8 assay. The results showed that the two compounds were identified as Aldisin and 2-Bromoaldisin which exhibited the potent inhibitory activities against Caco-2 , HCT116 , 7721 , U251 and A495 cells in human. The Aldisin showed the strongest cytotoxic activity against 7221 cell with IC50(67. 56±3. 06)μg/mL. The findings indicate that the extracts as natural products are considered as a potential anti-tumor candi-date for further study.%为进一步探求澳大利亚厚皮海绵Craniella australiensis在海洋药物研制中的作用,采用硅胶柱层析及Sephadex LH-20凝胶过滤层析等技术,从澳大利亚厚皮海绵乙酸乙酯层浸膏中分离纯化出两种化合物,并通过波谱数据与文献对照的方法对化合物进行了结构鉴定,采用CCK-8法对2个化合物进行体外抗肿瘤细胞活性试验。结果表明：从澳大利亚厚皮海绵分离得到的2个化合物分别为Aldisin和2-Bromoaldisin；体外抗肿瘤细胞活性试验显示,化合物Aldisin和2-Bromoaldisin对人克隆结肠腺癌细胞( Caco-2)、人结肠癌细胞(HCT116)、人肝癌细胞(7721)、人胶质瘤细胞(U251)和人肾癌细胞(A498)均具有抑制活性的作用,其中化合物Aldisin处理7721细胞的IC50为(67.56±3.06)μg/mL,抑制作用显著。研究表明,两种化合物属于天然产物,可作为潜在的抗肿瘤药物进行进一步研究。