目的：观察晚期氧化蛋白产物（ advanced oxidation protein product ，AOPP）对内皮细胞表达基质细胞衍生因子-1α（stromal cell derived factor -1α，SDF-1α）的影响，并探讨其作用机制。方法 ECV304细胞分别经0，50，100，200μmol/L AOPP处理后，用RT-PCR法检测其SDF-1αmRNA的表达，观察AOPP对ECV304细胞的SDF-1α表达的影响。 ECV304细胞先经NF-κB通路特异性阻断剂BAY11-7082处理，再经AOPP处理，用RT-PCR法检测其SDF-1αmRNA的表达，观察BAY11-7082对AOPP促ECV304细胞SDF-1αmRNA表达作用的影响。结果 AOPP促进ECV304细胞SDF-1αmRNA 表达，并且随AOPP浓度的升高，SDF-1αmRNA 的表达增加。对照组、AOPP 50μmol/L组、AOPP 100μmol/L组及AOPP 200μmol/L组SDF-1αmRNA/GAPDH mRNA值分别为0．034±0．005、0．109±0．019、0．226±0．037及0．322±0．043，组间比较差异均有显著性意义，P＜0．05。BAY11-7082抑制AOPP促ECV304细胞SDF-1αmRNA表达，两组细胞SDF-1αmRNA/GAPDH mRNA值分别为0．256±0．035和0．322±0．043，P＜0．05。结论 AOPP可上调ECV304细胞表达SDF-1α，NF-κB通路可能是介导这一作用的重要途径之一。%Objective To observe the effect of advanced oxidation protein product ( AOPP) on the expression of SDF-1αin ECV304 cells,and to investigate the possible mechanism .Methods SDF-1αmRNA expressions were revealed by RT -PCR respectively in ECV304 cells preincubated with different concentrations of AOPP .SDF-1αmRNA expression was re-vealed by RT-PCR in ECV304 cells preincubated with BAY 11-7082 which was a inhibitor of NF -κB signal transduc-tion pathway .Results SDF-1αexpression was constitutionally detected in ECV 304 cells and was up-regulated by AOPP in a concentration-dependent manner .The promoting effect of AOPP on the expression of SDF -1αin ECV304 cells could be inhibited by BAY11-7082.Conclusion AOPP could up-regulate SDF-1αexpression in ECV304 cells by NF-κB signal transduction pathway .
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