安体舒通对糖尿病大鼠肾脏 Nephrin蛋白表达的影响

摘要

Objective To observe the effects of spironolactone on the expression of nephrin in the kidney of diabetic ne -phrophathy rats.Methods Healthy male Sprague-Dawley(SD)rats were randomly divided into normal control (NC) and experimental groups .Rat models of diabetic nephropathy were established by intraperitoneal injection of streptozotocin ;After the modeling, the experimental group were randomly divided into diabetic nephrophathy ( DN) group and spironolactone group.At the end of 12th week, blood urea nitrogen(BUN), creatinine clearance rate(Ccr), 24h urine protein(24hUpro) and lipoprotein A were determined , the expression level of Nephrin protein was observed by western blot .Results Com-pared with spironolactone groups , BUN, Ccr,24Upro and lipoprotein A in DN group statistically increased;the expression level of nephrin protein in spironolactone group was increased compared with DN group .Conclusion Spironolactone can im-prove the renal function of rats with diabetic nephropathy .Its mechanism may be ralited to the increasing of nephrin expres-sion.%目的：探讨安体舒通对糖尿病（ DM）大鼠肾脏Nephrin蛋白表达的影响。方法 SD大鼠分为对照组、糖尿病肾病（DN）组、安体舒通组。腹腔注射链脲佐菌素（50 mg/kg）建立糖尿病大鼠模型，实验12周末检测肾功能、蛋白尿、脂蛋白，Western Blot检测足细胞Nephrin 蛋白含量（P＜0．05）。结果较安体舒通组，DN组大鼠尿素氮（BUN）、血肌酐（Ccr）下降，24 h尿微量白蛋白排泄率（UAER）增多，脂蛋白升高，且肾脏Nephrin蛋白表达下调，差异有显著性意义（P＜0．05）。结论安体舒通对糖尿病肾病肾功能有保护作用，其机制可能与其上调Nephrin蛋白有关。