目的：探讨大鼠急性脊髓损伤（ SCI ）程度与血清β－淀粉样前体蛋白（β－APP ）和超敏C反应蛋白（ hs－CRP）含量变化的相关性。方法取雌性SD大鼠90只，采用动脉夹压力法制备大鼠脊髓轻度损伤（轻度损伤组，30只）和重度损伤（重度损伤组，30只）动物模型，另30只大鼠仅行椎板切除作为假手术组。通过行为学观察（ BBB评分）了解脊髓损伤程度及变化，分别于造模成功后12 h、24 h、3 d、7 d、14 d采股静脉血，ELISA检测不同程度SCI大鼠伤后血清β－APP、hs－CRP含量变化。结果重度脊髓损伤组与轻度损伤、假手术组各时间点BBB评分差异有显著性意义（ P＜0．05）。β－APP于损伤后24 h达到高峰后逐渐回落，hs－CRP于3 d达到高峰后回落。重度脊髓损伤组与轻度脊髓损伤、假手术组各时间点β－APP、hs－CRP比较差异均有显著性意义（ P＜0．05）；轻度脊髓损伤组与假手术组β－APP、hs－CRP水平除14 d点差异无显著性意义外，其余各时间点差异均有显著性意义（P＜0．05）。结论急性SCI大鼠血清β－APP、hs－CRP水平变化可反映SCI的严重程度，β－APP比hs－CRP的变化敏感。%Objective To investigate the relation to the degree of the acute spinal cord injury ( SCI) by detecting the serumβ-amyloid precursor protein (β-APP) and hypersensitive-cross-reactive protein ( hs-CRP) levels after acute spinal cord injury in rate at different time points.Methods Ninety healthy adult female SD rats were randomly assigned into 3 groups.The bulldog clamp was used to create models of severe SCI ( group A) and mild SCI ( group B) .In group C, only laminectomy was performed without SCI.The ethological study ( BBB scores) was used to learn the degree of the acute spi-nal cord injury.The serum was collected at 12 h, 24 h, 3 d, 7 d, and 14 d after operation through femoral vein.Serumβ-APP and hs-CRP were detected by ELISA.Results The serumβ-APP and hs-CRP reached the peak at 24 h and 3 d, respectively after acute SCI.Serumβ-APP and hs-CRP levels in SCI groups ( A and B) were both significantly higher than those in without SCI group ( C) at all time points ( P<0.05) , except at 14 d between group B and group C. Conclusions The serumβ-APP and hs-CRP levels are positively associated with the severity of SCI.β-APP would be a more sensitive indicator than hs-CRP in a certain time.A acute SCI model of SD rats can be established by bulldog clamp, in order to mimic the clinical SCI.
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