凋亡细胞及过氧化物酶体增殖物激活受体γ在子痫前期患者胎盘中的表达及临床意义

摘要

Objective To investigate the clinical significance of apoptosis index ( AI) and PPARγexpression in the placenta of patients with preeclampsia.Methods The placenta tissues from normal-term pregnancy, light preeclampsia and severe preeclampsia patients (30 cases in each group) were collected from 2014-2016 years in Dalian Municipal Women and Children,s Medical Center.TUNEL was performed to measure AI and immunohistochemistry ( S-P meth-od) was used to detect PPARγexpression in intermediate trophoblast of placental floor.Results AI was 1.5860 ± 0.2541, 5.2609 ±2.8643 and 9.0000 ±6.2763 in normal-term pregnancy group, the light preeclampsia group and the severe preeclampsia group, respectively (P<0.05).The PPARγexpression frequency was 46.7%, 93.3% and 100%in normal-term pregnancy group, the light preeclampsia group and the severe preeclampsia group, respectively (P<0.05).The AI was positively correlated with PPARγexpression in placenta of patients with preeclampsia (P<0.05).Conclusion PPARγcould involve in the pathogenesis of preeclampsia by inducing apoptosis of placental troph-oblast cells.%目的：研究中间型滋养细胞的凋亡及过氧化物酶体增殖物激活受体γ（ PPARγ）在子痫前期患者胎盘中的表达及临床意义。方法收集2014－2016年间大连市妇女儿童医疗中心住院分娩的正常妊娠晚期（正常组）、轻度子痫前期（轻度子痫前期组）及重度子痫前期（重度子痫前期组）产妇胎盘组织，各30例。采用TUNEL法检测3组胎盘底板中间型滋养细胞的凋亡并计算凋亡指数（AI），采用免疫组化S－P法检测PPARγ的表达情况。结果正常妊娠晚期组、轻度子痫前期组及重度子痫前期组胎盘滋养细胞的AI分别为1．5860±0．2541、5．2609±2．8643及9．0000±6．2763，呈增高趋势，各组间差异有显著性意义（ P＜0．05）。 PPARγ在正常妊娠晚期组、轻度子痫前期组及重度子痫前期组的表达阳性率分别为46．7％，93．3％，100％，呈增强趋势，各组间差异有显著性意义（ P＜0．05）。子痫前期胎盘组织中PPARγ与凋亡细胞的表达呈正相关（ r＝0．591， P＜0．05）。结论 PPARγ可能通过诱导胎盘滋养细胞的凋亡参与子痫前期的发病。