Methods for determining a position of the disease in a patient, a situation apoptotic cells in a sample, and a situation of a C u00e1 NCER in a patient.

摘要

Methods for determining a situation of disease in a patient, a situation apoptotic cells in a sample, and a situation of a C u00e1 NCER in one patient.The invention provides a method to determine a position of the disease in a patient by measuring the levels of expression of intracellular complexes are selected.In one aspect of the invention,The situation of the activation of apoptotic routes in a sample of the patient is determined by measuring relative amounts of protein are complex protein that are characteristic of the paths apoptu00f3tICOS.In particular,The invention provides a method to determine the situation of activation of mitochondrial apoptotic pathway by measuring, at the same time, relative amounts of complexes between proteins and protein 14-3-3Bad, on the one hand, and complexes of proteins Bcl-2 and bad protein, on the other side.Preferably,The methods of the invention are implemented using sets are binding compounds having releasable molecular labels that are specific for multiple components of one or more complex formadThe inside of apoptotic routes. After the connection, the molecular labels are released and separated from the test mixture for analysis.