氨肽酶N在乳腺癌中的表达及与MMP-2、MMP-9的相关性研究

摘要

目的 研究氨肽酶N(aminopeptidase N,APN)在乳腺癌中的表达及临床意义,并观察其与基质金属蛋白酶-2(matrix metalloproteinase-2,MMP-2)、基质金属蛋白酶-9(matrix metalloproteinase-9,MMP-9)的关系.方法 收集河北省人民医院病理科2011年7月至2012年9月乳腺癌石蜡标本60例,癌旁正常乳腺组织石蜡标本20例,及患者的临床病理资料.采用免疫组织化学SP法检测APN在80例标本中的表达,并分析APN表达与乳腺癌患者临床病理特征之间的关系.检测MMP-2、MMP-9在乳腺癌组织中的表达,及与APN表达的相关性.结果 APN在乳腺癌中的阳性表达率36.7％ (22/60)明显高于癌旁正常乳腺组织0％(0/20)(x2=10.115,P=0.001).APN在乳腺癌中的表达与肿瘤病理类型有关(P =0.035),多表达于浸润性导管癌;与患者绝经状况、肿瘤大小、临床分期、组织学分级、淋巴结转移状态、ER、PR、Her-2等无关(P＞0.05).乳腺癌组织APN的表达与MMP-2、MMP-9的表达无相关性(P＞0.05).结论 APN在乳腺癌中的表达高于正常乳腺组织,但APN在乳腺癌中侵袭转移的机制有待进一步深入研究.%Objective To investigate the significance of Aminopeptidase N (APN) expression in breast cancer,and to discuss the role of APN in the process of breast carcinoma invasion and metastasis by analyzing the relationships between APN and Matrix metalloproteinase-2 (MMP-2),Matrix metalloproteinase-9 (MMP-9).Methods Immunohistochemistry (SP method) was performed to detect the expressions of APN,MMP-2 and MMP-9 in 60 cases of breast cancer tissues,as well as APN expression in 20 cases of adjacent normal breast tissues.Results The positive frequency of APN expression in breast cancer tissues (36.7％,22/60) was significantly higher than that in adjacent normal breast tissues (0％,0/20) (x2 =10.115,P =0.001).APN expression was associated with tumor pathological types (P =0.035).However,there was no relationship between the APN expression and other clinicopathological features,such as menopausal state,clinical stage,tumor size,histological grade,nodal status,ER,PR,and Her-2 (P ＞0.05).There was no significant relationship between the APN expression and MMP-2,MMP-9 expression (P ＞ 0.05).Conclusion APN expression was higher in breast cancer than that in normal breast tissues,but it needs further study if APN expression could play a role in invasion and metastasis in breast cancer.