外源性谷氨酰胺对机体抗氧化酶影响的实验研究

摘要

Objective To explore the influence of exogenous glutamine(Gln)on the antioxidant enzymes. Methods Twenty two rats (specific pathogen free) were randomly divided into experimental group(A),control group (B),and standard group(C). Animals of group A received gavage daily with alanyl-glutamine,group B with 0.9%normal saline,and group C were set up as control. Groups A and B were given gavage consecutively for 14 days. Blood specimens of rats of the three groups were detected for hydrogen peroxide enzyme(CAT), superoxide dismutase(SOD) and glutathione oxidation reductase(GSH-Px). Results The difference of CAT between groups A and C was statistically significant(P<0.05), while the level of CAT at 14d of experimental group was significantly higher than the standard group(P<0.05). The difference of GSH-Px between groups A and C was statistically significant(P<0.05), no difference between groups A and B was found(P>0.05).The level of GSH-Px at 14d of experimental group was remarkably higher than that of standard group(P<0.05). No significant difference of SOD was noted among all of the three groups (P>0.05). Conclusion Temperal administration of exogenous Gln may significantly increase CAT and GSH-Px levels, and serum SOD as well,thereby enhancing the ability to scavenge free radicals and antioxidant of the body.%目的：探讨外源性谷氨酰胺（Gln）对机体抗氧化酶的影响。方法选择无特定病原体成年大鼠22只，随机分为实验组（A）、对照组（B）、标准组（C），A组每日丙胺酰谷氨酰胺注射液灌胃，B组0.9%生理盐水灌胃，C组不灌胃；A、B两组灌服时间均为连续14 d，完毕后留取血样检测3组大鼠血清过氧化氢酶（CAT）、超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH-Px）活性，并予以统计分析。结果 CAT ：A、C组间差异有统计学意义（P<0.05），其余组间差异无统计学意义（P>0.05），实验组14 d时血清CAT水平显著高于标准组（P<0.05）；GSH-Px ：A、C组间差异有统计学意义（P<0.05），A与B组间差异无统计学意义（P>0.05），实验组14 d时血清GSH-Px水平显著高于标准组（P<0.05）；SOD ：各组间差异均无统计学意义（P>0.05）。结论短期外源性补充Gln可显著提高血清CAT及GSH-Px活性水平，并可提高血清SOD活性水平，提高机体清除自由基及抗氧化能力。