外源性 Vc 对普安银鲫仔鱼抗氧化酶活性的影响

摘要

The activities of antioxidant enzyme were determined during larva development of Carassius auratus gibelio, and effect of vitamin c was also measured .Vitamin C, with the concentration of 20, 25, 30 and 35mg/L, was added in the water when experiment conduct .The rate of egg hatching and larval survival was recorded .The activities of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-Px) were determined.The results showed that the activities of superoxide dismutase and catalase increased with the development of larva of Puan silver crucian carp , while the activity of glutathion peroxidase decreased .30 mg/L Vitamin C solution could improve the activities of superoxide dis -mutase, catalase and glutathion peroxidase significantly .The activity of superoxide dismutase decreased after 72 hours (P<0.05), while the activities of catalase and glutathion peroxidase had no significant change (P >0.05).The research indicated that superoxide dismutase and catalase played priority role during larva development of Puan silver crucian carp . Appropriate vitamin C solution could improve the antioxidant capacity of larva .%将维生素 C 添加到普安银鲫的孵化水体中，维生素 C 浓度设置为20、25、30和35 mg／L，记录孵化率及仔鱼存活率，测定各浓度组中普安银鲫（Carassius auratus gibelio）仔鱼发育过程中超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和谷胱甘肽过氧化物酶（GSH－Px）的活性，研究维生素 C（Vc）对普安银鲫仔鱼发育的影响。结果显示： SOD 和 CAT 的活性随着普安银鲫仔鱼发育时间的延长而增加，而 GSH－Px 活性则随着发育时间的延长而降低；30 mg／L 的 Vc 溶液能显著（P ＜0．05）提高普安银鲫仔鱼 SOD、 CAT 和 GSH－Px 活性，且 SOD 的活性在出膜72 h 后开始显著降低（P ＜0．05），而 CAT 和 GSH－Px 则无显著变化（P ＞0．05）。结果表明：普安银鲫仔鱼中抗氧化作用以 SOD 和 CAT 为主；适宜浓度的 Vc 添加到普安银鲫的育苗水体中能提高仔鱼的抗氧化能力。