在水温24℃下测定了普安银鲫胚胎发育过程中超氧化物歧化酶、过氧化氢酶和谷胱甘肽过氧化物酶的活性。试验结果表明，超氧化物歧化酶、过氧化氢酶和谷胱甘肽过氧化物酶在成熟卵中具有活性，分别为（10．213±0．458） U／mg、（8．701±0．371） U／mg和（55．485±4．896） U／mg ，属母源性抗氧化酶。对照组从成熟卵发育至出膜前期，超氧化物歧化酶和谷胱甘肽过氧化物酶活性逐渐升高，而过氧化氢酶的活性呈“升高-降低-升高”的趋势，在心跳期降到最低，为（6．175±0．532） U／m g ，随后其活性开始上升。30 m g／L的维生素C组，普安银鲫胚胎从受精卵至出膜前期，超氧化物歧化酶、过氧化氢酶和谷胱甘肽过氧化物酶活性总体上高于对照组。胚胎发育过程中母源性抗氧化酶发挥重要作用；孵化水体中适宜质量浓度的维生素C提高了胚胎期的抗氧化能力。%T he activities of superoxide dismutase (SOD ) ,catalase (CA T ) and glutathion peroxidase and effects of exogenous vitamin C on these enzymatic activities were studied during embryonic development of crucian carp Carassius auratus at water temperature of 24 ℃ .The results showed that there were activities of SOD[(10 .213 ± 0 .458) U/mg] ,CAT [(8 .701 ± 0 .371) U/mg] and glutathion peroxidase [(55 .485 ± 4 .896) U/mg] ,being derived from maternal antioxidant enzymes .In the control group ,the activities of SOD and glutathion peroxidase were increased gradually from mature eggs to pre-hatching ,while the activ-ity of CAT showed a tend of up-down-up ,the minimum [(6 .175 ± 0 .532) U/mg] in heart beating stage , and then elevated .There were higher activities of SOD ,CAT and glutathion peroxidase from mature egg to pre-hatching in the embryos exposed to vitamin C solution at a rate of 30 mg/L than that in the control group .Maternal antioxidative enzymes was found to play an important role during embryonic development of the crucian carp and appropriate concentration of vitamin C in the water used during the embryonic de-velopment led to improve the antioxidant capacity in the embryos of the crucian carp .
展开▼
