血浆外泌体上调bFGF促体外微血管网形成的研究

摘要

Objective:This study was aimed to explore the effect of exosome from plasma to angiogenesis in endothe-lial cells( EC) in vitro. Methods:Exosome isolated from rabbit plasma was used to cultivate EC,observe the tube network for-mation,and detect the expression of basic fibroblast growth factor( bFGF) and vascular endothelial growth factor( VEGF) . Re-sults:Compared to control group,the experimental group had more tube network formation,higher expression and secretion of bFGF at 72 h,respectively(P <0. 001,P <0. 01). No statistical difference was found when comparing the expression of VEGF(P>0. 05). Conclusion:Exosome from plasma has up-regulation effect to bFGF and can promote angiogenesis in EC.%目的:探索血浆来源的外泌体对血管内皮细胞(endothelial cells,EC)微血管生成的体外调控作用.方法:提取家兔血浆外泌体用于培养EC,观察体外微血管网的形成,并检测碱性成纤维细胞生长因子(basic fibroblast growth factor,bFGF)和血管内皮生长因子(vascular endothelial growth factor,VEGF)的表达.结果:加入外泌体的实验组相比对照组,体外微血管网状结构数目增多,72 h时bFGF的表达量和分泌量更高(P<0.001,P<0.01),VEGF的表达量差异无统计学意义(P>0.05).结论:血浆外泌体具有上调bFGF并促进EC微血管生成的作用.