[Objective] To explore the expression of BRAF in the mucosa of benign and malignant colorectal cancer and its correlation with clinical pathology. [Methods] Immunohistochemistry DAB method was used to detect the expression of BRAF in 42 cases of colorectal cancer, 10 cases of hyperplastic polyp and 18 cases of colorectal adenoma. One-factor analysis of variance and x2 test were used to analyze the correlation between gender, age, tumor size, differentiation, invasion or lymph node metastasis and the expression of BRAF. [Results]The positive rate of BRAF in hyperplastic polyp, colorectal adenoma and colorectal cancer increased progressively, and there were significant differences among groups ( P ＜0.05). With the increasing of atypical hyperplasia, the positive rate of BRAF elevated gradually( P ＜0.05). The positive rate of BRAF was negatively correlated with tumor differentiation. The positive rate of BRAF in tissues with lymph node metastasis was significantly higher than that in tissues without lymph node metastasis( P ＜0.05). With the increasing of the differentiation, the mean methylation level of BRAF elevated markedly( P ＜0.05). The mean methylation level of BRAF in tissues with lymph node metastasis was higher than that in tissues without lymph node metastasis( P ＜0.05). [Conclusion] The expression of BRAF has obvious correlation with tumor differentiation and lymph node metastasis, but has no relation with gender, age, tumor size and invasion. BRAF has an important value in the diagnosis and treatment of colorectal cancer.%[目的]探讨BRAF在良恶性结直肠肿瘤黏膜中的表达及其与临床病理关系.[方法]使用免疫组化DAB显色的方法检测BRAF在42例结直肠癌样品、10例增生性息肉样品和18例结直肠腺瘤的表达情况,采用单因素方差分析和x2检验来比较患者性别、年龄、肿瘤大小、分化程度、浸润深度及有无淋巴结转移与BRAF表达的阳性率之间的相关性.[结果]BRAF在增生性息肉、结直肠腺瘤和结直肠癌中,其阳性率递增,且组间比较具有统计学差异(P<0.05);且不典型增生程度的上升,BRAF的阳性率也随着逐步升高(P<0.05); BRAF的阳性率与肿瘤分化程度呈负相关,有淋巴结转移的组织BRAF的阳性率显著高于没有淋巴结转移的组织(P<0.05);分化程度由低到高,BRAF基因的平均甲基化水平显著升高(P<0.05);淋巴结转移的组织BRAF平均甲基化水平高于未转移的组织(P<0.05).[结论]BRAF蛋白的表达与肿瘤分化、淋巴结转移情况呈明显相关性,与患者的性别、年龄、肿瘤大小和浸润深度没有相关性,对结直肠癌的诊断和针对性治疗具有重要的参考价值.
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