谷氨酸受体拮抗剂对电休克后抑郁大鼠记忆障碍的影响

摘要

[目的]探讨谷氨酸(Glu)受体拮抗剂对电休克后抑郁大鼠记忆障碍的影响.[方法]健康雄性SD大鼠24只,随机分为空白组、电休克(ECT)组、地卓西平马来酸盐(MK-801)+ECT组(联合组),每组大鼠8只.空白组大鼠尾静脉注射生理盐水2 mL,ECT组建立大鼠ECT模型,联合组ECT前在大鼠尾静脉注射2 mL MK-801.采用Morris水迷宫学习记忆试验探索大鼠记忆障碍,高效液相色谱法测定大鼠脑内Glu和γ-氨基丁酸(GABA)水平并比较.[结果]ECT组和联合组大鼠逃避潜伏期较空白组明显延长,且有统计学差异(t=7.708、3.408,P＜0.05);联合组大鼠逃避潜伏期明显短于ECT组,且有统计学差异(t=5.137,P＜0.05).ECT组和联合组大鼠游泳百分率、穿过原平台位置次数较空白组明显减少,且有统计学差异(t=12.039、4.886,P＜0.05);联合组大鼠游泳百分率、穿过原平台位置次数明显高于ECT组,且有统计学差异(t=7.543,P＜0.05).ECT组和联合组大鼠脑内Glu和GABA含量较空白组明显升高,且有统计学差异(ECT组:t=6.701、5.639,联合组:t=2.630、2.233,P＜0.05);联合组大鼠脑内Glu和GABA含量明显低于ECT组,且有统计学差异(t=4.789、3.881,P＜0.05).[结论]Glu受体拮抗剂MK-801可减轻电休克后抑郁大鼠记忆障碍,且可降低脑内Glu和GABA含量,提示MK-801可能通过降低脑内Glu和GABA水平,改善其认知功能.%[Objective] To investigate the effect of glutamate (Glu) receptor antagonist on memory impairment in rats after electroconvulsive shock (ECT).[Methods]A total of 24 healthy male SD rats were randomly divided into the blank group,ECT group and MK-801+ECT group,with 8 rats in each group.Rats in the blank group were injected with normal saline 2 mL,and rats in the ECT group were treated with ECT.Rats in the MK-801+ECT group were injected with 2 mL MK-801 into their tail veins before ECT.Morris water maze learning and memory tests were used to explore memory impairment in rats,and the content of Glu and GABA in rat brain was determined by HPLC.[Results]The escape latency of ECT and MK-801+ECT groups was significantly longer than that of the blank group (t =7.708,3.408,P ＜0.05),and the escape latency of the MK-801+ECT group was significantly shorter than that of ECT group;there was statistical difference (t =5.137,P ＜0.05).The percentage of swimming rats in the ECT and MK-801+ ECT groups decreased significantly compared to the control group (t =12.039,4.886,P ＜0.05).However,the percentage of swimming rats in the MK-801+ECT group was significantly higher than that in the ECT group,and the difference was statistically significant (t =7.543,P ＜0.05).The number of rats passing through the original platform in the ECT and MK-801 +-ECT groups decreased significantly compared to that in the blank group (t =9.459,2.156,P ＜0.05).In addition,the number of rats passing through the original platform in the MK-801 + ECT group was significantly higher than that in the ECT group,and there was statistical difference (t =9.508,P ＜0.05).Glu and GABA content levels in the ECT and MK-801 +ECT groups rat brain were significantly higher than those in the blank group,and there were significant differences (ECT group:t =6.701,5.639,MK-801+ECT group:t =2.630,2.233,P ＜0.05).The Glu and GABA contents in the brain of rats in the MK-801+ECT group were significantly lower than in the ECT group,and there was statistical difference (t =4.789,3.881,P ＜0.05).[Conclusion]Glu receptor antagonist MK-801 can alleviate the memory impairment in rats after electroconvulsive therapy as well as reduce the content of Glu and GABA in the brain,which has important research significance.